吉首大学学报（自然科学版）2025，Vol.46Issue(3)：62-74,13.DOI:10.13438/j.cnki.jdzk.2025.03.008
有毒药用植物野八角叶绿体基因组特征及系统发育分析
Characterization and Phylogenetic Analysis of the Chloroplast Genome of the Toxic Medicinal Plant Illicium simonsii
摘要
Abstract
In order to explore the chloroplast genome characteristics of the toxic medicinal plant Illicium simonsii and analyze its relationships with other species of the Illicium,this study used next-generation sequencing(NGS)technology to perform high-throughput sequencing for leaf material of Illicium si-monsii,and obtained the complete chloroplast genome after assembly and annotation,which was analyzed using bioinformatics.Meanwhile,phylogenetic tree of Illicium simonsii and the related species in the Il-licium genus were constructed based on chloroplast genomes and coding sequences(CDS).The result showed that the full length of the Illicium simonsii chloroplast genome was 143 012 bp,which encoded 112 genes,including 78 protein-coding genes,29 tRNA genes,4 rRNA genes,and 1 pseudogene,with a total GC molar fraction of 39.1％;there were a total of 100 simple sequence repeats(SSRs)in the chlo-roplast genome of Illicium simonsii,with the largest number of mono-nucleotides.The degree of chlo-roplast genomic variation in Illicium plants all showed higher LSC and SSC regions than IRs regions,and the five highly variable regions were trnK-UUU-rps16,accD-psal,rpl20-clpP1,ycf1,atpF;Within the genus Illicium,Illicium simonsii was most closely related to Illicium burmanicum,with a support rate as high as 100％,and both Illicium simonsii and Illicium verum belong to the same monophyletic clade,exhibiting closer phylogenetic affinity.关键词
野八角/八角属/叶绿体基因组/系统发育/亲缘关系Key words
Illicium simonsii/Illicium/complete chloroplast genome/phylogeny/genetic relationship分类
生物学引用本文复制引用
高恬恬,毛玖阳,李海峰,张德全..有毒药用植物野八角叶绿体基因组特征及系统发育分析[J].吉首大学学报（自然科学版）,2025,46(3):62-74,13.基金项目
国家自然科学基金资助项目(32060091) （32060091）
云南省中青年学术和技术带头人后备人才项目(2105AC160063) （2105AC160063）
