吉首大学学报（自然科学版）2025，Vol.46Issue(3)：75-81,7.DOI:10.13438/j.cnki.jdzk.2025.03.009
基于响应面法的玉竹多糖脱蛋白工艺优化
Optimization of Deproteinization Process of Polysaccharides from Polygonatum odoratum Based on Response Surface Methodology
摘要
Abstract
Using Polygonatum odoratum(Mill.)Druce powder as the raw material,this study investigated the effects of chloroform to butyl alcohol volume ratio,Sevage reagent to polysaccharide sample volume ratio,and shaking time on polysaccharide retention rate and protein removal rate.Based on single factor experiments,the protein removal process for polysaccharides was optimized using response surface methodology.The optimal con-ditions were determined as follows:V(chloroform)∶V(butyl alcohol)=4∶1,V(Sevage reagent)∶V(Polysac-charide sample)=1∶2,and shaking time of 30 min.Under these conditions,the polysaccharide retention rate reached 76.01％,and the protein removal rate was 28.73％.This optimized protocol provides a reliable approach for efficiently removing proteins while preserving polysaccharide integrity during the purification of Polygonatum odoratum(Mill.)Druce polysaccharides.关键词
玉竹多糖/脱蛋白/Sevage方法/响应面法Key words
Polygonatum odoratum(Mill.)Druce polysaccharides/deproteinization/Sevage method/re-sponse surface分类
中医学引用本文复制引用
章扬,谢菲,陈淑婷,杜来红,胡珂美,邹彬,王琦,成江..基于响应面法的玉竹多糖脱蛋白工艺优化[J].吉首大学学报（自然科学版）,2025,46(3):75-81,7.基金项目
湖南省自然科学基金资助项目(2024JJ7409) （2024JJ7409）
国家大学生创新创业训练计划项目(202210531011) （202210531011）
吉首大学药学院创新性研究项目(2024年立项) （2024年立项）
