菌物学报2025，Vol.44Issue(6)：120-129,10.DOI:10.13346/j.mycosystema.240354
多脂鳞伞对四氯化碳诱导小鼠急性肝损伤的保护作用机制
Protective effects and functional mechanism of Pholiota adiposa against carbon tetrachloride-induced acute liver injury in mice
摘要
Abstract
Pholiota adiposa is an edible and medicinal fungus with various pharmacological effects.The protective effects and functional mechanism of ethanol extract from P.adiposa(EPA)on acute liver injury induced by CCl4 in BALB/c mice were studied.The liver organ index of mice was measured.The content of alanine aminotransferase(ALT),aspartate aminotransferase(AST),tumor necrosis factor α(TNF-α),and interleukin 6(IL-6)in serum and the content of superoxide dismutase(SOD),reducing glutathione(GSH)and malondialdehyde(MDA)in liver were measured by ELISA.H&E staining and TUNEL apoptosis assay were used to analyze the liver histopathological changes,and the apoptosis of liver cells was analyzed by immunohistochemistry analysis and Western blot.The results showed that after EPA administration,the liver index of mice was significantly decreased,and the content of ALT,AST,TNF-α and IL-6 in serum of treated group were significantly decreased as compared with that of model control(MC)group.The content of SOD and GSH in liver homogenate were significantly increased,and the content of MDA was significantly decreased.As a result,the oxidative stress of liver was improved.The results of H&E staining and TUNEL assay showed that the liver cell swelling induced by CCl4 was significantly improved after EPA administration,and the apoptosis rate of liver cells was also significantly reduced.Immunohistochemical analysis and Western blot analysis showed that EPA could significantly inhibit the overexpression of apoptosis proteins such as BAX,Caspase3 and Cleaved Caspase3,and reduce the level of Bcl-2 protein,showing significant protective effect on CCl4-induced apoptosis of liver cells.关键词
多脂鳞伞/肝损伤保护机制/生化指标/免疫组化/蛋白质印迹Key words
Pholiota adiposa/protective mechanism of liver injury/biochemical index/immunohistology/Western blot引用本文复制引用
王晓岩,刘岩,贺智杰,谈中玉..多脂鳞伞对四氯化碳诱导小鼠急性肝损伤的保护作用机制[J].菌物学报,2025,44(6):120-129,10.基金项目
吉林省科技厅青年成长科技计划(20220508074RC)This work was supported by the Jilin Province Science and Technology Department Project of the Youth Growth(20220508074RC). （20220508074RC）
