菌物学报2025，Vol.44Issue(6)：130-144,15.DOI:10.13346/j.mycosystema.250017
桑黄多糖对溃疡性结肠炎的干预作用
The intervention effects of Sanghuangporus sanghuang polysaccharides on ulcerative colitis
摘要
Abstract
Ulcerative colitis(UC)is a chronic inflammatory bowel disease protractedly and severely impacting the lives of patients.Polysaccharides extracted from Sanghuangporus sanghuang fruiting bodies and a mouse model of colitis was established using dextran sulfate sodium(DSS),and then intervention with S.sanghuang polysaccharides(PPs)was carried out.The weight,colon length,inflammatory factor levels,and oxidative stress levels of mice were detected;colon tissue sections were observed and changes in colon microbiota were analyzed to evaluate the intervention effect of PPs on colitis.The results show that PPs(400 mg/kg/d)is non-toxic to mice and can effectively slow down the weight loss and inhibit colon shortening,and reduce the structural damage and pathological changes of colon tissue in UC-suffering mice.Compared with mice administered with 3.5%DSS by gavage,mice intervened with PPs(400 mg/kg/d)showed decrease of myeloperoxidase(MPO)and pro-inflammatory factors interleukin-6(IL-6)and tumor necrosis factor α(TNF-α)content,and increase of total superoxide dismutase(T-SOD)content and anti-inflammatory factor interleukin-10(IL-10)content in colon tissue.To a certain extent,PPs can regulate the changes of gut microbiota structure induced by DSS in UC mice and enhance the diversity of gut microbiota.The abundance changes of Bacteroidetes,Firmicutes,Verrucomicrobia and Proteobacteria induced by DSS are improved by PPs,which can effectively inhibit the expression of pro-inflammatory factors and cellular oxidative stress in colitis mice,thereby reducing intestinal inflammatory response,alleviating intestinal histological changes,enriching the diversity and regulating the abundance of gut microbiota,and thus alleviating UC symptoms.关键词
桑黄多糖/溃疡性结肠炎/炎症因子/氧化应激/肠道菌群Key words
Sanghuangporus sanghuang polysaccharides/ulcerative colitis/inflammatory factor/oxidative stress/gut microbiota引用本文复制引用
许瀛引,林杨,彭坤,董茜,张谦,舒雪琴,张仕林,周洁,谢丽源,王勇,彭卫红..桑黄多糖对溃疡性结肠炎的干预作用[J].菌物学报,2025,44(6):130-144,15.基金项目
四川省财政自主创新专项(2022ZZCX096) （2022ZZCX096）
四川省农业科学院"1+9"揭榜挂帅项目(1+9KJGG007) （1+9KJGG007）
四川省科技厅项目(2023NSFSC1664)This work was supported by the Sichuan Province Financial Independent Innovation Project(2022ZZCX096),the Sichuan Academy of Agricultural Sciences"1+9"Key Project(1+9KJGG007),and the Sichuan Province Science and Technology Department Project(2023NSFSC1664). （2023NSFSC1664）
