解放军医学杂志2025，Vol.50Issue(5)：592-598,7.DOI:10.11855/j.issn.0577-7402.0165.2024.1125
中强静磁场暴露对小鼠情绪行为及脑损伤相关分子的影响
Effects of moderate static magnetic field exposure on emotional behavior and brain damage related molecules in mice
王雪佳 1杨雪枫 2叶雨萌 2王勇懿 2郝延辉 2左红艳 2柳峰松 3李杨1
作者信息
- 1. 河北大学生命科学学院,河北 保定 071002||军事科学院军事医学研究院辐射医学研究所,北京 100850
- 2. 军事科学院军事医学研究院辐射医学研究所,北京 100850
- 3. 河北大学生命科学学院,河北 保定 071002
- 折叠
摘要
Abstract
Objective To investigate the effects of a 100 mT static magnetic field(SMF)on emotional behavior and brain damage-related molecules in mice.Methods Fifty-eight C57BL/6N mice were randomly divided into control group(n=25)and observation group(n=33).Mice in observation group were exposed to a 100 mT SMF for 0.5 h/d over 14 consecutive days,while mice in control group underwent pseudo-exposure.On the 7 and 14 days of exposure,anxiety-like behavior was assessed using open field and elevated plus maze tests.Cerebral blood flow was monitored using laser speckle imaging,and the levels of tumor necrosis factor-α(TNF-α),interleukin(IL)-1β,IL-4,central nervous system specific protein β(S100β),neuron-specific enolase(NSE),and brain-derived neurotrophic factor(BDNF)were measured by radioimmunoassay.BDNF expression in the brain was detected by immunofluorescence.Results On the 7 and 14 days of SMF exposure,the open field and elevated plus maze tests showed no statistically significant differences between observation and control groups in the frequencies,durations,and distance entering the central area of the open field and the open arm of the elevated plus maze(P>0.05).Laser speckle imaging revealed no significant difference in cerebral cortical perfusion compared with pre-exposure period(P>0.05).The results of radioimmunoassay showed that compared with control group,on the 7 d of SMF exposure,the serum IL-1β,NSE and S100β levels were significantly increased(P<0.05),the serum BDNF level was significantly decreased(P<0.05),and the IL-1β and TNF-α contents in brain tissues were significantly increased in observation group(P<0.01).On the 14 d of SMF exposure,serum IL-1β,TNF-α,NSE,and S100β levels were significantly increased(P<0.05,P<0.0001),and the brain IL-1β and TNF-α levels were significantly increased(P<0.01)in observation group.No statistically significant differences were found in anti-inflammatory cytokine IL-4 level of serum and brain tissue or BDNF content of brain tissue between the two groups(P>0.05).Conclusion Continuous exposure to a 100 mT SMF for 14 d at 0.5 h/d induces neuroinflammation and brain damage in mice,without inducing anxiety-like behavior.关键词
静磁场/焦虑/血流/脑损伤/炎症Key words
static magnetic field/anxiety/blood flow/brain injury/inflammation分类
医药卫生引用本文复制引用
王雪佳,杨雪枫,叶雨萌,王勇懿,郝延辉,左红艳,柳峰松,李杨..中强静磁场暴露对小鼠情绪行为及脑损伤相关分子的影响[J].解放军医学杂志,2025,50(5):592-598,7.
