分析测试学报2025，Vol.44Issue(6)：1055-1065,11.DOI:10.12452/j.fxcsxb.240912392
基于香气强度和活度值的贵州烟叶蜜甜香特征风味成分分析
Analysis of Flavour Components of Honey-sweet Aroma Characteristics of Guizhou Tobacco Based on Aroma Intensity and Activity Value
摘要
Abstract
In order to understand the characteristic aroma components of honey-sweet-flavor tobacco smoke from different producing areas in Guizhou,the volatile compounds of 9 smoke samples were identified and analyzed using cambridge filter trapping combined with gas chromatography-olfactome-ter-mass spectrometry(GC-O-MS).Multivariate statistical methods combined with relative odor ac-tivity value(ROAV)were used to screen the characteristic aroma components of honey-sweet aroma in tobacco smoke,and the intrinsic material basis of the honey-sweet flavor of Guizhou tobacco was ex-plored.The results showed that GC-MS identified a total of 144 volatile components,including 31 ketones,22 hydrocarbons,22 heterocyclics,22 phenols,15 esters,13 acids,11 alcohols,6 al-dehydes and 2 other components.A total of 69 aroma compounds were identified by GC-O-MS,and the aroma characteristics mainly showed fruity,milky,caramelized,floral,nutty and honey-sweet aromas,and 25 aroma-active compounds(ROAV≥1)such as 1-penten-3-one,benzyl acetate,guai-acol,and ethyl-damascenone were screened out.Through orthogonal partial least squares discrimi-nant analysis(OPLS-DA)analysis,a total of 34 key differential volatile aroma compounds with VIP>1 were screened.Combined with the conditions of VIP>1(P＜0.05)and ROAV≥1,8 characteristic aroma components were obtained,including 1-penten-3-one,methylcyclopentenolone,ethylcyclo-pentenolone,p-isopropylphenol,eugenol,indole,trans-nerolidol and ethyl-damascenone.The partial least squares regression(PLSR)results further verified that these 8 aroma substances had an important contribution to the formation of the overall aroma profile and were the key aroma compounds of the sweet aroma of Guizhou tobacco leaves.This study used GC-O-MS technology,ROAV value and multivariate statistical methods to screen out the key aroma substances in the sweet aroma of to-bacco smoke,providing a reference for clarifying the aroma characteristics of tobacco smoke in Gui-zhou.关键词
贵州烟叶/蜜甜香/气相色谱-嗅闻仪-质谱仪/相对香气活度值/多元统计分析/特征致香物Key words
Guizhou tobacco/honey-sweet aroma/gas chromatography-olfactometer-mass spec-trometry/relative odor activity value/multivariate statistical analysis/characteristic odorant分类
化学化工引用本文复制引用
罗俊华,龙勇,汪雪娇,蔡凯,林叶春,赵瑞娟,孙振春,曹建新..基于香气强度和活度值的贵州烟叶蜜甜香特征风味成分分析[J].分析测试学报,2025,44(6):1055-1065,11.基金项目
贵州省科技厅基础研究计划项目(黔科合基础-ZK[2021]一般175) （黔科合基础-ZK[2021]一般175）
中国烟草总公司贵州省公司科技项目(2023XM19) （2023XM19）
贵阳市生态食品(风味与品质)产业技术创新中心 （风味与品质）
贵阳贵安2023年科技人才培养计划(筑科项目[2024]2-5号) （筑科项目[2024]2-5号）
