实用临床医药杂志2025，Vol.29Issue(9)：7-11,17,6.DOI:10.7619/jcmp.20243844
长链非编码RNA X染色体失活特异转录本和微小RNA-410-3p在类风湿关节炎患者血清中的表达及意义
Expression and significance of long non-coding RNA X-inactive specific transcript and microRNA-410-3p in serum of patients with rheumatoid arthritis
摘要
Abstract
Objective To investigate the expression of long non-coding RNA(lncRNA)X-inac-tive specific transcript(XIST)and microRNA-410-3p(miR-410-3p)in serum of patients with rheu-matoid arthritis(RA),and their correlation with bone mineral density(BMD)and bone metabolism indicators.Methods A total of 109 RA patients were selected as research subjects.After measuring BMD using dual-energy X-ray absorptiometry(DXA),they were divided into osteoporosis group(53 cases)and non-osteoporosis group(56 cases).BMD at different sites and serum levels of lncRNA XIST,miR-410-3p,and bone metabolism indicators[β-C-terminal telopeptide of type Ⅰ collagen(β-CTX),parathyroid hormone(PTH),osteocalcin(OC),osteoprotegerin(OPG),and N-terminal propeptide of type Ⅰ procollagen(PⅠNP)]were compared between the two groups.Pearson correlation analysis was used to analyze the correlation between lncRNA XIST and miR-410-3p,as well as their correlation with BMD and bone metabolism indicators.Logistic regression analysis was used to screen the influencing factors for osteoporosis in RA patients.Results Compared with the non-oste-oporosis group,the osteoporosis group had lower BMD at the lumbar spine,femoral neck,greater trochanter,and Ward's triangle area,higher levels of OPG,β-CTX,and lncRNA XIST,and lower levels of mi R-410-3p,with statistically significant differences(P＜0.05).There were no statistical-ly significant differences in PTH,OC,and PⅠNP levels between the two groups(P＞0.05).Pear-son correlation analysis showed that serum lncRNA XIST levels were negatively correlated with miR-410-3p levels(r=-0.435,P＜0.001).Serum lncRNA XIST levels were negatively correlated with BMD at the lumbar spine,femoral neck,greater trochanter,and Ward's triangle area(P＜0.05),and positively correlated with OC levels(P＜0.05).Serum mniR-410-3p levels were positively correlated with BMD at the lumbar spine,femoral neck,greater trochanter,and Ward's triangle area(P＜0.05),and negatively correlated with OC levels(P＜0.05).Multivariate logistic regression analysis showed that BMD,lncRNA XIST,and miR-410-3p were all independent influencing factors for osteoporosis in RA patients(P＜0.05).Conclusion LncRNA XIST expression is upregulated in serum of RA patients with osteoporosis,while mi R-410-3p expression is downregulated.They are closely related to BMD at the lumbar spine,femoral neck,greater trochanter,and Ward's triangle area,as well as OC levels of bone metabolism indicators.关键词
微小RNA-410-3p/长链非编码RNA/X染色体失活特异转录本/类风湿关节炎/骨密度/骨代谢/骨钙素/骨质疏松症Key words
microRNA-410-3p/long non-coding RNA/X-inactive specific transcript/rheu-matoid arthritis/bone mineral density/bone metabolism/osteocalcin/osteoporosis分类
医药卫生引用本文复制引用
吴海镕,孙承军..长链非编码RNA X染色体失活特异转录本和微小RNA-410-3p在类风湿关节炎患者血清中的表达及意义[J].实用临床医药杂志,2025,29(9):7-11,17,6.基金项目
湖北省武汉市医学科研项目(WX21D83) （WX21D83）
