菌物学报2025，Vol.44Issue(6)：15-29,15.DOI:10.13346/j.mycosystema.250020
血红色钉菇菌塘土壤微生物群落多样性
Microbial community diversity of mushroom-producing soil of Chroogomphus rutilus
摘要
Abstract
Mushroom-producing soil is the important place for the fruiting body formation of mycorrhizal edible fungi.Microorganisms that can promote the mycorrhizal formation exist in the mushroom-producing soil,so the study of microbial communities of the mushroom-producing soil is crucial for understanding the mycorrhizal formation.In this study,the composition of microbial communities and diversity of the mushroom-producing soil of Chroogomphus rutilus and non-mushroom-producing soil were clarified by use of amplicon sequencing.The results showed that the Chao 1,Observed species,Shannon,Simpson and Pielou indices of bacteria and fungi in the non-mushroom-producing soil were significantly higher than those in the mushroom-producing soil(P<0.05).The dominant phyla in both the mushroom-producing and non-mushroom-producing soil are Proteobacteria and Basidiomycota.In the mushroom-producing soil,the relative abundance of Proteobacteria,Ascomycota and Rozellomycota is greater than that in the non-mushroom-producing soil,among them the relative abundance of Rozellomycota is 2.94 times higher than that in the non-mushroom-producing soil.The dominant genera in the mushroom-producing and non-mushroom-producing soil are Russula and Suillus.In the mushroom-producing soil,the relative abundance of Leptodontidium is 1.27 times higher than that in the non-mushroom-producing soil,the relative abundance of Penicillium is 4.47 times higher than that in the non-mushroom-producing soil,the relative abundance of Suillus is 5.72 times higher than that in the non mushroom-producing soil,the relative abundance of Oidiodendron is 6.31 times higher than that in the non-mushroom-producing soil,the relative abundance of Talaromyces is 18.14 times higher than that in the non-mushroom-producing soil,and the genus Chroogomphus and Sebacina only exists in the mushroom-producing soil.LEfSe analysis showed that 26 different taxonomic levels of fungi(1 phylum,1 class,4 orders,6 families,9 genera,5 species)and 20 different taxonomic levels of bacterial indicator groups(2 phyla,3 classes,3 orders,6 families,6 genera)were obtained in the mushroom-producing soil.The co-occurrence network analysis showed that there was no significant difference in the number of nodes and edges of fungal and bacterial communities between the mushroom-producing and non-mushroom-producing soil.Chroogomphus rutilus was positively correlated with the abundance of unidentified bacterium ASV20(uncultured Proteobacteria)and that of unidentified fungus ASV22(Tomentella),and the correlation was high.The results provide reference for the artificial domestication and cultivation of C.rutilus.关键词
血红色钉菇/菌塘土壤/高通量测序/微生物群落多样性Key words
Chroogomphus rutilus/mushroom-producing soil/high-throughput sequencing/microbial community diversity引用本文复制引用
王兴红,边禄森,刘慧慧,马庆华,陈淋,苗朔..血红色钉菇菌塘土壤微生物群落多样性[J].菌物学报,2025,44(6):15-29,15.基金项目
中国林业科学研究院中央级公益性科研院所基本科研业务费专项资金(CAFYBB2022ZC006)This work was supported by the Fundamental Research Funds for the Central Non-profit Research Institution of Chinese Academy of Forestry(CAFYBB2022ZC006). （CAFYBB2022ZC006）
