菌物学报2025，Vol.44Issue(6)：55-72,18.DOI:10.13346/j.mycosystema.250030
海南霸王岭三种植被类型下血叶兰根内和根区土壤中共生真菌多样性及其种子原位共生萌发初探
Diversity of symbiotic fungi in roots and rhizosphere soil of Ludisia discolor in three vegetation types in Bawangling area of Hainan and isolation of germination-enhancing fungi through in situ baiting experiment
摘要
Abstract
Comparative analysis of fungal community composition and diversity in root and rhizosphere soil samples of traditional medicinal plant Ludisia discolor growing in three vegetation types(tropical valley rainforest,tropical lowland rainforest,and the transitional zone between the two vegetation types)in the Bawangling area of Hainan's tropical rainforest is conducted.Isolation of germination-enhancing fungi is also carried out through in situ baiting experiment to explore the specific orchid mycorrhizal fungal(OMF)resources in the three vegetation types.There are significant differences in the symbiotic fungal community composition between the root and rhizosphere soil samples.Fungal amplicon sequence variants(ASVs)in the rhizosphere soil decrease and those in the roots increase with the transitions of vegetation types from tropical valley rainforest to tropical lowland rainforest.Ceratobasidiaceae and Sebacinaceae,both specific to orchids,were found to be abundant in the root and rhizosphere soil,but the highest abundance was found in the roots.Ceratobasidium species were identified using in situ baiting technique.In indoor germination experiments,(32±4)d after inoculation with the fungal strain,the seed embryos significantly enlarged to form protocorms,with an average germination rate of(30.75±5.14)%,while no germination was observed in the control group without fungal inoculation.This suggests that the isolated Ceratobasidium fungi can effectively promote seed germination of L.discolor.The results reveal the deep impact of environmental changes on the diversity of symbiotic fungi in L.discolor and provide evidence for screening symbiotic fungal species facilitating seed germination in artificial propagation of orchids.关键词
血叶兰/种子原位共生萌发/ITS扩增子测序/核心菌群/兰科植物保育Key words
Ludisia discolor/in situ baiting/ITS amplicon sequencing/core fungal communities/Orchidaceae conservation引用本文复制引用
兰烯,赵莹,罗天雄,宋希强,钟云芳,冯雪萍..海南霸王岭三种植被类型下血叶兰根内和根区土壤中共生真菌多样性及其种子原位共生萌发初探[J].菌物学报,2025,44(6):55-72,18.基金项目
海南省自然科学基金(322MS019,322QN229)This work was supported by the Hainan Province Natural Science Foundation(322MS019,322QN229). （322MS019,322QN229）
