菌物学报2025，Vol.44Issue(6)：73-84,12.DOI:10.13346/j.mycosystema.240341
山田胶锈菌性、锈孢子阶段效应蛋白的预测和筛选
Prediction and screening of Gymnosporangium yamadae effector proteins at spermogonial and aecial stages
摘要
Abstract
Effectors are essential for pathogens to manipulate plant immunity and colonize host.Elucidating the key effectors secreted by Gymnosporangium yamadae that regulate host defense responses is pivotal for understanding its pathogenic mechanism.In this study,100 candidate effectors were predicted based on the comparative transcriptome analysis of spermogonial and aecial stages of G.yamadae,combined with structural characteristics,and 43 fragments with accurate sequences were cloned.Transient expression in Nicotiana benthamiana showed that five candidate effectors(GyS-2,GyS-3,GyS-16,GyS-20,and GyA-4)triggerred cell death,while GyS-44 with a pathogenic domain had no impact on N.benthamiana's immune defense.Subcellular localization assays revealed that GyS-2 and GyS-44 were localized in the cytoplasm,GyA-4 aggregated around the chloroplasts,and GyS-3,GyS-16,and GyS-20 were localized in and around the nucleus.Yeast signal peptide secretion assays verified that the signal peptides of these six key effectors possessed secretory capabilities.The results provide a basis for further in-depth exploration of the molecular interactions between G.yamadae effectors and the host.关键词
效应子/异源表达/亚细胞定位/植物免疫Key words
effectors/heterologous expression/subcellular localization/plant immunity引用本文复制引用
金之鑫,张晓漫,梁英梅..山田胶锈菌性、锈孢子阶段效应蛋白的预测和筛选[J].菌物学报,2025,44(6):73-84,12.基金项目
国家自然科学基金(32371885,31870628)This work was supported by the National Natural Science Foundation of China(32371885,31870628). （32371885,31870628）
