辽宁农业科学Issue(3)：1-7,7.DOI:10.3969/j.issn.1002-1728.2025.03.001
大连软枣猕猴桃细菌性溃疡病鉴定及药剂筛选
Pathogen Identification and Bactericide Screening of Bacterial Canker Disease of Actinidia Arguta in Dalian
摘要
Abstract
Bacterial canker is a devastating disease in Actinidia arguta production.In order to identify the pathogen and screen the effective bactericide,in this study,disease tissues were collected from Jinzhou Dis-trict,Dalian,and used tissue isolation to isolate the pathogen,and identified the pathogen based on morpholo-gy,physiological and biochemical characteristics,16S rDNA sequence,and pathogenicity test.The optimal medium and temperature were determined,and the inhibition effects of twenty bactericide on the pathogen were also measured by using inhibition zone method.The results showed that the pathogen of bacterial canker of A.arguta in Dalian was Pseudomonas syringae pv.actinidiae biovar 2,the optimal medium for the pathogen was LB medium and the optimal temperature was 25℃.Nine agents,including tetramycin,ethylicin and bro-nopol,had antibacterial effect on the pathogen,and the combination of tetramycin and ethylicin at 5∶1 ratio has the best antibacterial effect.The results provide theoretical basis for identifying the pathogen of bacterial canker of A.arguta and using bactericide.关键词
软枣猕猴桃/细菌性溃疡病/丁香假单胞菌猕猴桃致病变种/药剂筛选Key words
Soft jujube kiwifruit/Bacterial ulcer disease/Pseudomonas syringae kiwifruit pathogenic variant/Medicament selection分类
植物保护学引用本文复制引用
郑泽洋,尤文忠,刘娟娟,孙俊,纪盈睿,朱可馨,李玉莹,陈喜忠,刘振盼..大连软枣猕猴桃细菌性溃疡病鉴定及药剂筛选[J].辽宁农业科学,2025,(3):1-7,7.基金项目
辽宁省应用基础研究计划项目(2022JH2/101300284) （2022JH2/101300284）
辽宁省农业科学院协同创新"揭榜挂帅"专项项目(2022XTCX0503) （2022XTCX0503）
