林业科学2025，Vol.61Issue(6)：109-119,11.DOI:10.11707/j.1001-7488.LYKX20240401
基于51K液相探针构建国外松良种的DNA指纹图谱
Construction of a DNA Fingerprinting of Elite Varieties of Introduced Exotic Pines in China Based on 51K Liquid-Phased Probes
摘要
Abstract
[Objective]Slash pine(Pinus elliottii),loblolly pine(P.taeda),Caribbean pine(P.caribaea)and their interspecific hybrids are the main timber species introduced and domesticated from North America in China.In recent years,dozens of elite varieties of foreign pines have been approved.As the morphological characteristics of exotic pines are very similar at seedling stage,it is a challenge to distinguish these elite varieties.The purpose of this study is to construct a set of DNA fingerprints for foreign pine varieties,which can be used for the identification of different foreign pine species,and provide technical support for the management of germplasm resources and intellectual property protection of exotic pine varieties.[Method]The 51K liquid phase probe microarray of slash pine and loblolly pine was used to capture SNPs of 38 elite varieties of exotic pines and their hybrids,and obtained their genotype data.The core SNPs in exotic pine germplasm resources were able to be efficiently identified through screening genetic parameters,such as minor allele frequency,deletion rate,heterozygosity and polymorphism information content.The number of SNP markers was further simplified based on PIC values to achieve the goal of efficiently identifying samples with a smaller number of SNPs.[Result]A total of 60 567 SNPs loci were obtained by SNP locus capture and genotyping.Through strict control of minor allele frequency,deletion rate,heterozygosity and other parameters,344 core SNPs were screened.The PIC value was further reduced to 20 SNPs,with which slash pine,loblolly pine,Caribbean pine and their hybrids were able to be efficiently distinguished.This approach was used to successfully establish DNA fingerprint profiles for 38 elite pine varieties.[Conclusion]In this study,a DNA fingerprinting system for elite exotic pine varieties has been developed,and a method for identifying exotic pines and their elite varieties has been established.The research results can provide technical support for the protection of intellectual property rights,germplasm management,and pedigree analysis of elite varieties.关键词
DNA指纹图谱/品种鉴别/湿地松/火炬松Key words
DNA fingerprinting/pine varieties identification/Pinus elliottii/P.taeda分类
农业科技引用本文复制引用
吴亚荻,刁姝,丁显印,黄琴韵,栾启福..基于51K液相探针构建国外松良种的DNA指纹图谱[J].林业科学,2025,61(6):109-119,11.基金项目
生物育种重大专项(2023ZD0405902,2023ZD04058) （2023ZD0405902,2023ZD04058）
国家重点研发专项(2022YFD2200023-2). （2022YFD2200023-2）
