临床输血与检验2025，Vol.27Issue(3)：306-309,4.DOI:10.3969/j.issn.1671-2587.2025.03.004
17例ABO血型鉴定中混合视野现象的分析及利用三代测序技术鉴定其等位基因
Analysis of Mixed Vision in 17 Cases of ABO Blood Group Typing and Identification of Their Alleles Using PacBio SMRT Third-generation Sequencing Technology
夏悦昕 1杨宜承 1李春祥 1周世航 1刘志远2
作者信息
- 1. 大连市血液中心
- 2. 大连市妇女儿童医疗中心(集团)中心实验室,辽宁 大连 116000
- 折叠
摘要
Abstract
Objective Using PacBio third-generation sequencing technology to determine the haplotype genotypes of 17 blood samples exhibiting mixed vision in ABO blood group typing,with the aim of accurately identifying the ABO blood types.Methods The ABO phenotype was identified by immunoserological method.The ABO genotype was identified by PacBio Third-Generation sequencing technology.Results Seventeen specimens exhibited subtype characteristics in the immunological serology tests.The results of third-generation gene sequencing technology showed that nine of them were subtypes,including five cases of A1Bweak,two cases of cisAB,one case of Bweak,and one case of B(A).The remaining eight cases were non-subtypes,comprising six cases of A1B,one case of A1,and one case of B.Conclusion By combining serological tests with gene sequencing technology to determine the genotype,we can accurately identify blood types and improve the safety of clinical blood transfusions.关键词
ABO基因分型/PacBio三代测序/混合视野Key words
ABO genotype/PacBio third-generation sequencing technology/Mixed Vision分类
临床医学引用本文复制引用
夏悦昕,杨宜承,李春祥,周世航,刘志远..17例ABO血型鉴定中混合视野现象的分析及利用三代测序技术鉴定其等位基因[J].临床输血与检验,2025,27(3):306-309,4.
