临床输血与检验2025，Vol.27Issue(3)：325-329,5.DOI:10.3969/j.issn.1671-2587.2025.03.008
利用细胞化学染色方法和原位杂交技术探讨红细胞中核酸物质表达及定位情况
Exploring the Expression and Localization of Nucleic Acid Substances in Red Blood Cells Using Cellular Chemical Staining Methods and in Situ Hybridization Techniques
摘要
Abstract
Objective To explore the expression and localization of nucleic acid substances in red blood cells(RBCs).Methods Firstly,healthy human RBCs were selected as experimental samples(A549 nucleated cell line as control).Cellular chemical staining methods and in situ hybridization techniques were used to detect DNA and RNA,and to clarify the expression of nucleic acid substances in RBCs.Secondly,We extracted microvesicles(MVs)derived from RBCs and co-cultured them with the highly phagocytic mouse monocyte macrophage line RAW264.7 to detect the localization of RBC specific miRNA(miR-451a)in the receptor cell RAW264.7 by in situ hybridization staining.Results Our test results show that there are nucleic acid substances(DNA and RNA)in RBCs.And there exists miR-451a with high expression levels in RBCs,miR-451a in RBCs may enter the receptor cells via MVs.Conclusion The presence of nucleic acid substances in RBCs can be detected using cellular chemical staining methods and in situ hybridization techniques.关键词
红细胞/核酸物质/miR-451a/细胞化学染色/原位杂交技术Key words
Red blood cells/Nucleic acid/miR-451a/Cellular chemical staining methods/In situ hybridization techniques分类
生物科学引用本文复制引用
孙莉萍,曹腾宇,段翠密,于洋..利用细胞化学染色方法和原位杂交技术探讨红细胞中核酸物质表达及定位情况[J].临床输血与检验,2025,27(3):325-329,5.基金项目
本课题受国家自然科学基金面上项目(No.81970167)资助 （No.81970167）
