南京中医药大学学报2025，Vol.41Issue(6)：794-803,10.DOI:10.14148/j.issn.1672-0482.2025.0794
莪术醇通过SREBP-1/FASN/EGFR轴延缓非小细胞肺癌进程
Curcumol Retards Progression of Non-Small Cell Lung Cancer through the SREBP-1/FASN/EGFR Pathway
摘要
Abstract
OBJECTIVE To investigate the effect of Curcumol on lipid metabolism in non-small cell lung cancer(NSCLC)and its related molecular mechanism.METHODS A nude mouse xenograft tumor model was established,and Curcumol was administered for 14 d.The volume and weight of subcutaneous tumors were recorded.Hematoxylin-eosin(HE)and immunohistochemical staining were used to observe the pathological morphology of tumor tissues and organs,as well as the expression levels of Ki67 protein.Bio-chemical kits were used to detect the levels of lipids in tumor tissues.Western blot was used to detect the expression levels of SREBP-1,FASN,and EGFR proteins in tumor tissues.The qPCR and immunofluorescence were used to detect the expression of SREBP-1 in tumor tissues.Curcumol was used to treat human NSCLC cell line A549 and A549 cells overexpressing SREBP-1.The EdU assay was used to detect the proliferation ability of lung cancer cells;the MTT colorimetric method was used to detect cell viability;the Transwell assay was used to detect tumor cell migration and invasion;flow cytometry was used to detect cell apoptosis;biochemical kits were used to detect lipid levels in cells;and Western blot was used to detect the expression levels of SREBP-1,FASN,and EGFR proteins.RE-SULTS In the in vivo experiment,compared with the control group,Curcumol significantly inhibited the growth of subcutaneous xen-ografts,inhibited tumor cell proliferation(P<0.05,P<0.01),reduced the levels of triglycerides(TG),total cholesterol(T-CHO),and free fatty acids(FFA)in tumor tissues(P<0.05,P<0.01),downregulated the expression of SREBP-1,FASN,and p-EGFR pro-teins(P<0.01),and inhibited the expression of SREBP-1 mRNA in tumor tissues(P<0.05,P<0.01).In the in vitro experiment,compared with the control group,Curcumol significantly inhibited the proliferation,migration,and invasion of A549 cells,reduced cell viability(P<0.05,P<0.01),promoted tumor cell apoptosis(P<0.01),downregulated the levels of FFA,T-CHO,and TG in A549 cells(P<0.05,P<0.01),and inhibited the expression of SREBP-1,FASN,and p-EGFR proteins(P<0.05,P<0.01).Additionally,overexpression of SREBP-1 antagonized the inhibitory effects of Curcumol on lipid synthesis and EGFR activation,and weakened the inhibitory effects of Curcumolc on tumor cells.CONCLUSION Curcumol inhibits the expression levels of SREBP-1 and FASN,re-duces lipid synthesis,blocks EGFR signal transduction,and slows down the occurrence and development of NSCLC.关键词
莪术醇/非小细胞肺癌/脂质代谢/固醇调节元件结合蛋白-1/脂肪酸合成酶/表皮生长因子受体Key words
Curcumol/non-small cell lung cancer/lipid metabolism/SREBP-1/FASN/EGFR分类
医药卫生引用本文复制引用
孟令隆,白丰玺,赵雨,吴晓宇,梁丽丽..莪术醇通过SREBP-1/FASN/EGFR轴延缓非小细胞肺癌进程[J].南京中医药大学学报,2025,41(6):794-803,10.基金项目
河南科技共建联合共建项目(LHGJ20190751) （LHGJ20190751）
