世界科学技术-中医药现代化2025，Vol.27Issue(5)：1244-1252,9.DOI:10.11842/wst.20240524004
不同时辰电针合谷即时效应和脑功能连接差异
Immediate Effects and Differences in Brain Functional Connectivity of Electroacupuncture at Hegu During Different Time Periods
摘要
Abstract
Objective To provide experimental evidence for selecting acupuncture timing in clinical practice,the optimal time for enhancing the brain effects of electroacupuncture at the Hegu acupoint(LI4)by observing brain imaging data,hemodynamic changes and differences in brain functional connectivity across the twelve traditional Chinese time periods were determined.Methods Thirty-six C57BL/6 mice were randomly divided into 12 groups corresponding to each of the twelve time periods(Zi,Chou,Yin,Mao,Chen,Si,Wu,Wei,Shen,You,Xu,Hai),with 3 mice per group.Each mouse received electroacupuncture stimulation using the same protocol.Brain imaging data and dynamic hemodynamic changes were collected using functional ultrasound imaging(FUS)ultrasound imaging technology every 0.4 s over a total duration of 420 s,covering pre-acupuncture(resting state),during acupuncture(task state),and post-acupuncture(post-task state)phases.The hippocampal region(HIP)was used as the observation point to analyze changes in functional connectivity between HIP and other brain regions before and after acupuncture.Results Compared to other time periods,the Mao group exhibited the largest whole-brain activation area and the highest average activation signal intensity.The hemodynamic signal increase in the hippocampal region was more pronounced,and the post-acupuncture blood flow signal intensity remained significantly higher than the pre-acupuncture resting state.Functional connectivity data revealed that,using 0.2 as the standard value,the Mao group showed the greatest number of altered brain regions before and after acupuncture.Notably,only in the Mao group was there a significant enhancement in connectivity between the bilateral hippocampal regions.Conclusion The immediate effects of electroacupuncture at the Hegu acupoint(LI4)and brain functional connectivity vary significantly across different time periods,aligning with the traditional Chinese medicine theory of meridian qi and blood flow.Mao time is identified as the optimal period.关键词
时间针灸/气血流注/子午流注/合谷/海马/脑血流动力学/脑功能连接Key words
Chrono-acupuncture/Qi and blood flow/Zi Wu Liu Zhu/Hegu acupoint(LI4)/Hippocampus/Cerebral hemodynamics/Brain functional connectivity分类
医药卫生引用本文复制引用
许淑鱼,张帅,刘欣媛,李蔚娴,孙国杰,田青,杜艳军..不同时辰电针合谷即时效应和脑功能连接差异[J].世界科学技术-中医药现代化,2025,27(5):1244-1252,9.基金项目
国家自然科学基金委员会面上项目(82375464):电针调节IL-3/IL-3Rα介导AC-MG交互以促小胶质细胞吞噬Aβ机制研究,负责人:杜艳军 （82375464）
国家自然科学基金面上项目(82074566):基于p300/CBP/CREB通路研究电针调控阿尔茨海默病小胶质细胞M2/M1极化表型平衡的组蛋白乙酰化机制,负责人:杜艳军 （82074566）
湖北省联合基金重点项目(2023AFD112):"益肾调督"法电针调控AC-MG交互串扰以促AD脑内稳态平衡的机制研究,负责人:杜艳军. （2023AFD112）
