世界科学技术-中医药现代化2025，Vol.27Issue(5)：1267-1276,10.DOI:10.11842/wst.20240716004
电针耳甲区改善急性应激障碍小鼠抑郁样行为的机制研究
Study on the Mechanism of Action of Auricular Electroacupuncture on Depression-Like Behaviors in Acute Stress Disorder Mice
摘要
Abstract
Objective To observe the effects of electroacupuncture auricular acupuncture points on depression-like behaviors and ventral dorsal tegmental area(VTA)TH,DRD1 and DRD2 induced in well acute stress disorder(ASD)mice.Methods Experiment 1:18 male C57 mice were divided into 9 control and 9 model mice.Behavioural paradigms such as forced swimming test(FST)and tail suspension test(TST)were used to detect depression-like behaviors in the mice starting on day 5 and day 36;double-labelled staining for immediate early gene protein(C-Fos)and tyrosine hydroxylase(TH)was detected in the VTA of the mice by immunofluorescence.Experiment 2:45 male C57 mice were divided into 15 mice each in the control groups,model groups and electroacupuncture groups.30 days after completion of the electroacupuncture intervention,the mice were tested for depressive behaviors using behavioural paradigms such as FST and TST;dopamine receptor D1(DRD1)and dopamine receptor D2(DRD2)was detected in the VTA of mice in each group using fluorescence quantitative PCR and Western blot techniques;the number of TH neurons in the brain area of the VTA of mice was observed by immunofluorescence.Results Experiment 1:Compared with the control group,the immobile duration of the model group increased in the TST(P<0.001)and FST(P<0.001)on day 5 and day 6,and the immobile duration of the model group increased in the TST(P<0.01)and FST(P<0.01)on day 36 and day 37;compared with the control group,the number of C-Fos and TH fluorescence double-labelled neurons in the model group increased(P<0.05).Experiment 2:At day after completion of the electroacupuncture intervention,the immobile duration of TST(P<0.01)and FST(P<0.01)were decreased in the electroacupuncture group compared with the model group;compared with the control group,DRD1 mRNA(P<0.001)and protein expression(P<0.01)were increased in the VTA of the electroacupuncture group,and DRD2 mRNA(P<0.001)and protein expression(P<0.05)were decreased,TH protein expression(P<0.05)increased,and the number of TH neurons increased(P<0.01).Conclusion Early intervention with auricular electroacupuncture points has an ameliorative effect on depression-like behaviors induced by acute stress disorder in mice,which may be achieved by modulating the activity level of dopaminergic neurons and the expression level of DRD1 and DRD2 in the VTA.关键词
电针耳甲区/急性应激障碍/抑郁样行为/酪氨酸羟化酶/腹侧背盖区/多巴胺系统Key words
Electroacupuncture at auricular points/Acute stress disorder/Depression-like behaviors/Tyrosine hydroxylase/Ventral tegmental area/Dopamine system分类
医药卫生引用本文复制引用
张轩宇,韩静,林丽,时钰甦,施琳玉,李永丰..电针耳甲区改善急性应激障碍小鼠抑郁样行为的机制研究[J].世界科学技术-中医药现代化,2025,27(5):1267-1276,10.基金项目
咸阳市重点研发计划课题(S2024-ZDYF-SF-3089):针刺耳甲区改善急性应激障碍导致的抑郁样情绪作用机制研究,负责人:李永丰 （S2024-ZDYF-SF-3089）
陕西省教育科学规划领导小组办公室教育科学规划课题(SGH23Y2416):探究针灸推拿学研究生的创新思维与科学研究方法的培养与实践策略,负责人:李永丰 （SGH23Y2416）
陕西省科学技术厅一般项目(青年)(2022JQ-949):针刺通过调节MRN-VH的5-HT能投射改善不可控应激诱发的焦虑情绪机制研究,负责人:李永丰. （青年）
