山东农业科学2025，Vol.57Issue(5)：46-53,8.DOI:10.14083/j.issn.1001-4942.2025.05.005
亚精胺提高烟草幼苗抗盐性的转录组分析
Transcriptomic Analysis of Salt Tolerance Enhancement of Tobacco Seedlings by Spermidine
摘要
Abstract
In order to explore the alleviating effect of spermidine on salt stress to tobacco seedlings,Yun-yan 87 was used as material to study the effects of exogenous spermidine on physiological and biochemical characteristics and gene expression of tobacco seedlings under NaCl stress.The results showed that exogenous spermidine alleviated the inhibition effect of salt stress on tobacco seedlings,compared with NaCl treatment,the leaf water content,plant dry weight and chlorophyll content increased by 21.60%,18.48%and 59.75%,respectively.Spermidine significantly increased superoxide dismutase(SOD)and peroxidase(POD)activities and proline content of tobacco seedlings by 26.38%,39.09%and 32.67%,respectively,compared with NaCl treatment.A total of 4 339 differentially expressed genes regulated by Spd were screened out by transcriptomic analysis,which were mainly significantly enriched in the items of photosynthesis,secondary metabolite biosyn-thesis and carbohydrate metabolism and so on.This study clarified the application effect of spermidine on allevia-ting tobacco salt stress and screened out a series of key genes and metabolic pathways,which could provide the the-oretical reference and technical support for rational application of spermidine in flue-cured tobacco production.关键词
烟草/盐胁迫/亚精胺/转录组分析Key words
Tobacco/Salt stress/Spermidine/Transcriptomic analysis分类
农业科学引用本文复制引用
王文超,许嘉阳,田嘉树,刘腾飞,赵源,贾玮,许自成,张斌,崔光周..亚精胺提高烟草幼苗抗盐性的转录组分析[J].山东农业科学,2025,57(5):46-53,8.基金项目
河南省烟草公司信阳市公司科技项目(2022411500200092) （2022411500200092）
河南农业大学青年英才项目(30500999) （30500999）
