山东农业科学2025，Vol.57Issue(5)：64-68,74,6.DOI:10.14083/j.issn.1001-4942.2025.05.007
希尔斯山羊草细胞遗传学标记的建立
Establishment of Cytogenetic Marker for Aegilops searsii
摘要
Abstract
Aegilops searsii,a related plant of wheat,has excellent resistance to abiotic and biotic stres-ses,which is an excellent gene source for wheat improvement.Fluorescence in situ hybridization(FISH)is a powerful cytogenetic tool for detecting the chromosomes of some related plants of wheat.In order to verify its feasibility to identify the chromosomes of A.searii in wheat background,the FISH analysis of the Chinese Spring-A.searsii 1Ss to 7Ss addition lines was performed with Oligo-pSc119.2-1,Oligo-pTa535-1 and(GAA)8 as probes,and using Chinese Spring as control.The results of double-color FISH with Oligo-pSc119.2-1 and Oligo-pTa535-1 as probes showed that Oligo-pSc119.2-1 had hybridization signals at ends or proximal ends of the long and short arms of chromosomes in A.searii 1Ss to 7Ss,whereas Oligo-pTa535-1 had relatively weak hybridization signals only at near middle of 3Ss L and near terminal of 7Ss S.The result of FISH using(GAA)8 as probe showed that the hybridization signal was present in different positions on chromosomes 1Ss to 7Ss.At the same time,the ratio of long arm to short arm of each chromosome in A.searsii was analyzed and the results showed that the ratio of 1Ss to 7Ss chromosomes were different,among which,1Ss to 5Ss were subcentral cen-tromere chromosomes,and 6Ss and 7Ss were central centromere chromosomes.Therefore,combined with chro-mosome arm ratio,the FISH hybridization signal of Oligo-pSc119.2-1 or(GAA)8 could not only accurately distinguish each chromosome of A.searsii,but also be used as cytogenetic marker to identify the chromosome of A.searsii in wheat background.关键词
小麦/希尔斯山羊草/附加系/荧光原位杂交/染色体臂比Key words
Wheat/Aegilops searsii/Addition line/Fluorescence in situ hybridization(FISH)/Chromo-some arm ratio分类
农业科学引用本文复制引用
丁洪科,宫文萍,倪佳俊,王开,崔法,刘建军,刘成..希尔斯山羊草细胞遗传学标记的建立[J].山东农业科学,2025,57(5):64-68,74,6.基金项目
国家重点研发计划项目(2023YFD1201005) （2023YFD1201005）
山东省重点研发计划项目(2022LZG002-4) （2022LZG002-4）
山东省小麦产业技术体系项目(SDAIT-01-01) （SDAIT-01-01）
济南市"新高校20条"项目(202228067) （202228067）
