上海预防医学2025，Vol.37Issue(4)：332-335,4.DOI:10.19428/j.cnki.sjpm.2025.24780
上海市长宁区8例猴痘病毒基因组特征分析
Genomic characteristics of monkeypox virus from 8 cases in Changning District,Shanghai
摘要
Abstract
[Objective]To investigate the epidemiological feature of Mpox infection and genetic characteristics of Mpox viruses(MPXVs),so as to understand the etiological evolution of the pathogen.[Methods]The cases infected with MPXVs were originated from Changning District,Shanghai from July 20 to August 24 in 2023.Epidemiological investigations were conducted,and throat swabs,anal swabs,or vesicle fluid were collected for MPXVs nucleic acid testing.High-throughput sequencing was performed using Miniseq of the Illumina sequencing platform,and thereafter the sequences were concatenated and analyzed using the online analysis tool Nextclade.An evolutionary tree was constructed using the MEGA 11 software.[Results]All 8 cases were male,with an average age of(35.76±7.00)years.Among them,6 cases were identified through active hospital visits,and 2 cases were discovered during contact tracing for Mpox cases.Within the 21 days preceding the disease onset,all cases had male-male sexual behaviors,and the incubation period ranged from 6 to 10 days.3 cases had a history of sexually transmitted diseases(STDs).MPXVs nucleic acid testing indicated that the detection rate of MPXVs was found to be 25.00%for throat swabs,87.50%for anal swabs,and 100.00%for vesicle fluid,with statistically significant differences(χ2=11.052,P=0.004).Sequencing analyses using the online tool Nextclade indicated that all 8 MPXVs belonged to the West African clade Ⅱb,4 MPXVs were classified as C.1 sub-lineages,and 4 MPXVs were identified as C.1.1 sub-lineages.Phylogenetic analysis using MEGA 11 indicated that 5 MPXVs were classified as Lineage C.1.1,closely related to the prevalent strains in Portugal and other European regions.[Conclusion]The MPXVs sequences from Changning District are clssified into clade Ⅱb,lineage C.1.1.The detection rates of vesicle fluid and anal swabs for MPXVs are significantly higher than that of throat swabs.关键词
猴痘/猴痘病毒/基因特征Key words
monkeypox/monkeypox virus/genomic characteristic分类
医药卫生引用本文复制引用
何晓定,徐阳,尹宁,王震宇,郭家胤..上海市长宁区8例猴痘病毒基因组特征分析[J].上海预防医学,2025,37(4):332-335,4.基金项目
上海市长宁区急性传染病防制硕博士创新人才基地(RCJD2022S09) （RCJD2022S09）
上海市长宁区卫生健康委员会公共卫生特色专科项目(20232012) （20232012）
