中国森林病虫2025，Vol.44Issue(3)：50-59,10.DOI:10.19688/j.cnki.issn1671-0886.20250013
基于响应面法的金龟子绿僵菌M14-1菌株固体发酵培养条件优化
Optimization of solid fermentation conditions of Metarhizium anisopliae M14-1 based on response surface methodology
摘要
Abstract
To optimize solid fermentation conditions of Metarhizium anisopliae M14-1,the biocontrol fungus against of Monochamus saltuarius,and improve fermentation efficiency and spore yield,single factor experiment was used to determine parameters of solid medium and optimal culture conditions for strain M14-1,and important influencing factors were screened by Plackett-Burman experiment.On the basis,response surface method was used to optimize the important factors.The results showed that optimal conditions for solid fermentation of M.anisopliae M14-1 were as follows:mass ratio of rice flour to rice husk was 1.52∶1.95,glucose 0.10%,ammonium sulphate 0.10%,magnesium sulfate 0.10%,water content 80%,loading amount 100 g,inoculation amount 15%,fermentation temperature 27.18℃,fermentation time for 13 d.Spore production of strain M14-1 was 1.29 billion spores/g after optimization,but before optimization,spore production was 0.588 billion spores/g,and spore production effect was significantly improved.Solid fermentation conditions of strain M14-1 was optimized,and the results can provide theoretical basis for practical application.关键词
金龟子绿僵菌/单因素试验/Plackett-Burman试验/响应面法/固体发酵Key words
Metarhizium anisopliae/single factor experiment/Plackett-Burman experiment/response surface methodology/solid-state fermentation分类
林学引用本文复制引用
张瑶,邓勋,李亚洲,宋小双,杜文胜..基于响应面法的金龟子绿僵菌M14-1菌株固体发酵培养条件优化[J].中国森林病虫,2025,44(3):50-59,10.基金项目
黑龙江省重点研发计划项目"黑龙江省松林重大病虫害精准防控关键技术及应用"(2023ZX02B05) （2023ZX02B05）
黑龙江省科研院所科研业务费项目"黑龙江省松材线虫病入侵风险及防控关键技术"(CZKYF2021-2-B018) （CZKYF2021-2-B018）
黑龙江省森林保护学省级领军人才梯队后备带头人资助项目"云杉花墨天牛生防菌剂研发"(2023-547号) （2023-547号）
