实用医学杂志2025，Vol.41Issue(11)：1637-1644,8.DOI:10.3969/j.issn.1006-5725.2025.11.005
RAB2B通过NF-κB通路对胰腺癌增殖和转移的影响
The impact of RAB2B on pancreatic cancer proliferation and metastasis via the NF-κB pathway
摘要
Abstract
Objective To investigate the effects of Ras-related protein Rab-2B(RAB2B)on the biological behaviors of pancreatic cancer cells and elucidate its underlying mechanism.Methods PANC-1 cells,which exhibit relatively high RAB2B expression,and BXPC-3 cells,which display relatively low RAB2B expression,were selected from five pancreatic cancer cell lines.RAB2B-siRNA and pcDNA3.1-RAB2B plasmids were transfected into PANC-1 and BXPC-3 cells using a cell transfection technique.The CCK-8 assay was employed to evaluate the prolif-erative capacity of pancreatic cancer cells following RAB2B intervention.Wound healing and Transwell chamber assays were utilized to assess the migratory and invasive capabilities of pancreatic cancer cells.Additionally,the mRNA and protein expression levels of RAB2B,NF-κB,and Fibronectin 1(FN1)were analyzed by qRT-PCR and Western blot(WB),respectively.Results RAB2B mRNA and protein expression levels were significantly down-regulated in PANC-1 cells following transfection(P<0.05).CCK-8 assay results demonstrated that the proliferative capacity of PANC-1 cells was markedly reduced(P<0.05),and the wound-healing ability was substantially impaired(P<0.01)upon RAB2B knockdown.Transwell assays revealed a significant decrease in cell migration(P<0.01),while Western blot analysis indicated that the expression levels of phosphorylated p65 and FN1 were notably diminished(P<0.01).Conversely,overexpression of RAB2B reversed these aforementioned alterations.Conclusions Knockdown of RAB2B in PANC-1 cells significantly suppresses cell proliferation and migration,whereas overexpression of RAB2B in BXPC-3 cells markedly promotes these processes.This effect is likely mediated through the activation of the NF-κB signaling pathway and the subsequent regulation of FN1 expression.关键词
Ras-related protein Rab-2B/胰腺癌/NF-κB通路/纤维连接蛋白1/迁移/增殖Key words
Ras-related protein Rab-2B/pancreatic cancer/NF-κB pathway/fibronectin 1/transfer/proliferation分类
基础医学引用本文复制引用
李清,曾灵运,刘鑫,熊钰,宁静,覃山羽,陈秀秉..RAB2B通过NF-κB通路对胰腺癌增殖和转移的影响[J].实用医学杂志,2025,41(11):1637-1644,8.基金项目
国家自然科学基金项目(编号:81960439) （编号:81960439）
广西壮族自治区卫健委自筹项目(编号:Z-A20240981) （编号:Z-A20240981）
