邵娟 1梁钰 1郑雅婷 1曹有军 2陈洁 3钱赟达 3孔桂美4
作者信息
- 1. 225007,武警江苏总队扬州支队卫生队
- 2. 210019,南京医科大学附属明基医院放疗科
- 3. 314000,浙江中医药大学附属嘉兴市中医医院脾胃病科
- 4. 225009 扬州,江苏省人畜共患病重点实验室
- 折叠
摘要
Abstract
Objective To analyze the main active components in the serum of Banxia Xiexin decoction were analyzed,and its antibacterial effect in vitro.Methods After decocting and concentrating Banxia Xiexin decoction,the drug-containing serum of Banxia Xiexin decoction was prepared by intragastric administration to rats.The main drug active components in the serum were de-termined by high performance liquid chromatography-tandem mass spectrometry(HPLC-MS-MS).According to the results of HPLC-MS-MS results,the inhibitory effects of main active components and drug-containing serum of Banxia Xiexin decoction on Helico-bacter pylori and other bacteria were detected by microbroth dilution method in vitro.Results The test results showed that the con-tents of the main active components in the drug-containing serum,from high to low,were quercetin,isorhamnetin,baicalin,succinic acid,and codonopsis glycoside.The results of the in vitro antibacterial experiments showed that minimum inhibitory concentration(MIC)of codonopsis pilosula for inhibiting bacteria(staphylococcus aureus,staphylococcus albicans,escherichia coli,salmonella,proteus,helicobacter pylori)was 100µg/ml.,the MIC of isorhamulin was 400µg/ml for helicobacter pylori,and the remaining bacte-ria were 200µg/ml.The MIC of baicalin in helicobacter pylori was 400µg/ml,and others were 200µg/ml.The MIC of quercetin was 200 µg/ml.The MIC of drug containing serum was in a volume ratio of 1:4(staphylococcus aureus,escherichia coli and proteus)and 1:1(salmonella and helicobacter pylori).Succinic acid did not inhibit the growth of these bacteria.Conclusion The main ac-tive components of the drug-containing serum in the modified formula of Banxia Xiexin decoction,namely codonopsis glycoside,ba-icalin,quercetin and isorhamnine,have the effect of inhibiting helicobacter pylori and other bacteria,clarifying the main reason the main reasons for the antibacterial and anti-inflammatory effects of the modified formula of Banxia Xiexin decoction.关键词
半夏泻心汤/高效液相色谱-串联质谱/最小抑菌浓度/含药血清Key words
Banxia Xiexin decoction/high performance liquid chromatography tandem mass spectrometry/minimum inhibitory concentration/medicated serum分类
中医学
