山西农业大学学报（自然科学版）2025，Vol.45Issue(3)：10-20,11.DOI:10.13842/j.cnki.issn1671-8151.202411003
富氢水对水稻种子萌发的影响及其激素调节机制
Effects of hydrogen-rich water on rice seed germination and its hormonal regulation mechanism
摘要
Abstract
[Objective]Hydrogen-rich water(HRW)is a safe,green,and environmentally friendly new plant growth regulator.This article aimed to explore the effects of HRW seed soaking treatment on rice seed germination and seedling growth and pro-vide guidance for the application of hydrogen in agriculture.[Method]Using rice seeds(Oryza sativa L.japonica)as experimen-tal material,plump seeds were soaked in HRW at concentrations of 25％,50％,75％,and 100％for 8 h,with distilled water soaking as the control treatment(CK).The seeds were then placed in a thermostatically controlled environment(28℃,80％humidity,3000 lx,16:8 light/dark cycle)for 7 d.The plants were weighed daily and replenishment of sterile water.Germina-tion-related morphological traits,growth-related biochemical indices,hormone levels,and the expression of key genes in hor-mone signaling pathways were measured to explore the mechanism of the influence of hydrogen-rich water on rice germination.[Results](1)Compared with the CK,HRW soaking significantly increased rice shoot length,root length,germination index,and vigor index,with the 50％HRW treatment showing the most pronounced effects-40.71％,10.34％,33.73％,and 10.74％,respectively.(2)At day 3,the 50％HRW treatment significantly enhanced soluble protein,total soluble sugar,glu-cose,fructose,sucrose,auxin(IAA),and gibberellin(GA)contents while reducing soluble starch content.(3)Total sugars,fructose,and sucrose levels were significantly positively correlated with germination rate,whereas abscisic acid(ABA)content showed a significant negative correlation.[Conclusion]Hydrogen-rich water soaking promoted rice seed germination,with 50％concentration being optimal.HRW enhanced germination by regulating non-structural carbohydrate metabolism and hormone balance,breaking seed dormancy and improving metabolic activity.These findings highlighted HRW's potential as an effective agricultural agent for improving rice growth.关键词
富氢水/种子萌发/植物激素/非结构性碳水化合物Key words
Hydrogen-rich water/Seed germination/Plant hormones/Non-structural carbohydrates分类
农业科技引用本文复制引用
杨一凡,徐国辉,王文霞..富氢水对水稻种子萌发的影响及其激素调节机制[J].山西农业大学学报（自然科学版）,2025,45(3):10-20,11.基金项目
中国科学院战略重点研究计划(XDA28090307) （XDA28090307）
