刑事技术2025，Vol.50Issue(3)：279-284,6.DOI:10.16467/j.1008-3650.2024.0036
稀释血液血迹形成时间的研究
A Study on the Formation Time of Diluted Blood Stains
闫立强1
作者信息
- 1. 辽宁警察学院刑事科学技术系,辽宁大连 116038
- 折叠
摘要
Abstract
Rapid non-destructive testing of the formation time of blood stains at different dilution ratios under common temperature and humidity conditions was developed using UV visible reflectance spectroscopy.Dilute fresh blood with anticoagulant by 2,4,8,16,and 32 times with tap water.Take 20 μL diluted blood and drop it in the center of a white cotton cloth.After drying,use the reflection accessory of a UV visible spectrophotometer(UV-2450 type)to collect the reflection spectrum,and Excel was used to do regression analysis of trough values and formation time of blood stains at different concentrations.As time increased,there was a significant blue shift in the reflected wave troughs of normal and diluted blood stains near 577.0 nm and 540.0 nm.The values of R2 of the regression equation were all greater than 0.800 0,and there was a good linear relationship between the trough blue shift and the formation time of blood stain.The average speed of the trough blue shift increased with the increase of dilution ratio.Conclusion:The blue shift in the reflected wave valleys at 577.0 nm and 540.0 nm can serve as a basis for determining the formation time of normal blood stains,and combined with visual observation,it can also serve as a reference for determining the formation time of diluted blood stains.关键词
法医物证/紫外可见反射光谱/血迹/蓝移Key words
forensic evidence/UV visible reflectance spectrum/blood stains/blue shift分类
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闫立强..稀释血液血迹形成时间的研究[J].刑事技术,2025,50(3):279-284,6.
