刑事技术2025，Vol.50Issue(3)：285-290,6.DOI:10.16467/j.1008-3650.2024.0074
HS-GC-MS检测血液中五种常用含氟麻醉剂及六氟异丙醇
Determination of Five Common Fluorinated Anesthetics and Hexafluoroisopropanol in Blood Samples by HS-GC-MS
摘要
Abstract
This paper aims to establish a headspace(HS)gas chromatography-mass spectrometry(GC-MS)method for the detection of sevoflurane,desflurane,enflurane,isoflurane,halothane and hexafluoroisopropanol in biological samples.Blood samples(1.0 mL)and equivalent volume of deionized water were added into a 10 mL headspace vial,before the vial was sealed.The vial was extracted at 65 ℃ for 5 minutes and then the headspace was analyzed by GC-MS(EI),with the data being acquired under full scan mode.Sevoflurane,desflurane,enflurane,isoflurane,halothane and hexafluoroisopropanol showed good linear relationship between peak area and concentration within the investigated concentration ranges,with their coefficients of determination(R2)being over 0.9902.The lower limit of detection(LLOD)and limit of quantification(LOQ)for six analytes ranged 0.04-0.5 μg/mL and 0.06-1μg/mL,respectively.Their average recoveries among 61.19％—90.39％.The precision(RSD)of intra-day and inter-day behaved satisfied between 1.62％—17.29％and 0.85％—17.91％.The method is of high speed,simplicity,efficiency and low interference for determination of sevoflurane,desflurane,enflurane,isoflurane,halothane and hexafluoroisopropanol.关键词
含氟麻醉剂/法医毒物分析/顶空-气质联用仪Key words
fluorinated anesthetics/forensic toxicological analysis/HS-GC-MS分类
政治法律引用本文复制引用
马骥,张磊,方雅莉..HS-GC-MS检测血液中五种常用含氟麻醉剂及六氟异丙醇[J].刑事技术,2025,50(3):285-290,6.基金项目
广东省公安厅科技攻关项目(2022GDSSGG15、2022GDSSGG16) （2022GDSSGG15、2022GDSSGG16）
