烟草科技2025，Vol.58Issue(6)：9-18,10.DOI:10.16135/j.issn1002-0861.2025.0068
基于人肺上皮微流控芯片的烟气暴露毒理学评价方法
A method for assessment of cigarette smoke exposure toxicity based on human lung epithelial microfluidic chip
摘要
Abstract
To establish a reliable in vitro cigarette smoke exposure assessment model to study the toxicological effects of smoke exposure on human lung epithelial cells,a human lung epithelial microfluidic chip was designed and fabricated to simulate the airway-liquid interface,and smoke exposure analysis was performed based on the microfluidic chip.Cell viability,apoptosis and tight junction,calcium influx and gene expression were analyzed using immunofluorescence staining,flow cytometry and fluorescence quantitative PCR.To identify further biomarkers related to smoke stimulation,transcriptomic analysis using R language was performed on smoke stimulation-related RNA sequencing datasets(GSE186359 and GSE92662)and lung single-cell sequencing data from smokers and non-smokers(GSE131391),and experimental validation was also performed.The results showed that:1)Within 72 h after short-term smoke exposure,human lung epithelial BEAS-2B cells grew normally on microfluidic chips without apparent cell apoptosis or tight junction damage.2)Smoke exposure significantly increased calcium ion influx and upregulated the expression of the capsaicin receptor TRPV1/4,but did not induce the expression of pro-inflammatory factors.3)The smoke of three types of cigarettes with similar tar and nicotine deliveries but different sensory scores could all induce the expression of aldoketo reductase AKR1C2,and the expression level was positively correlated with the smoke stimulation score.The aldoketo reductase AKR1C2 gene may be a biomarker related to the level of smoke irritation.The developed smoke exposure assessment method based on the lung epithelial microfluidic chip provides a new research tool for evaluating the quality and toxicity of cigarettes.关键词
卷烟/烟气暴露/微流控芯片/肺上皮细胞/转录组学分析Key words
Cigarette/In vitro exposure/Microfluidic chip/Lung epithelial cell/Transcriptomic analysis分类
轻工业引用本文复制引用
潘晓薇,吴君章,陶红,邹正宇,王雨婷,任洁馨,陈森林..基于人肺上皮微流控芯片的烟气暴露毒理学评价方法[J].烟草科技,2025,58(6):9-18,10.基金项目
广东中烟工业有限责任公司科研项目"基于模拟肺呼吸道芯片的烟气暴露评价方法研究"(粤烟工[2021]科字第035号). （粤烟工[2021]科字第035号）
