烟草科技2025，Vol.58Issue(6)：19-27,9.DOI:10.16135/j.issn1002-0861.2024.0810
不同光质组合缓解烤烟新品系Hc400烟苗叶片花斑黄化的效果
Efficacy of different light quality combinations in alleviating mottled yellowing in leaves of a new flue-cured tobacco line Hc400 seedlings
摘要
Abstract
A new flue-cured tobacco line(coded Hc400 was developed by silencing the NtDHS gene in cv.K326)tends to exhibit mottled yellowing at the seedling stage.To address this issue and elucidate the effects of light quality on the growth of this flue-cured tobacco seedlings,K326 and Hc400 were selected as experimental subjects.Four combinations of light quality,high red light(red light∶blue light=3∶1),balanced light(red light∶blue light=1∶1),high blue light(red light∶blue light=1∶3)with white light as a control(illumination light),were established to investigate the impact of different light quality combinations on the growth and physiological characteristics of the flue-cured tobacco.The results showed that under the high red light treatment,the activities of phosphoribulokinase(PRK),ATP synthase,glyceraldehyde-3-phosphate dehydrogenase(GAPDH)and ribulose-1,5-bisphosphate carboxylase/oxygenase(Rubisco)in Hc400 leaves increased by 4.20%,41.13%,25.20%and 56.98%,respectively,compared to the control.The transpiration rate and net photosynthetic rate increased by 13.48%and 58.82%respectively and the contents of chlorophyll a,chlorophyll b,total chlorophyll and carotenoids increased by 54.07%,63.64%,56.84%and 59.09%respectively.In addition,the chloroplasts were more densely arranged and had a fuller and rounder shape.Under the high red light treatment,the chloroplasts of cv.K326 showed increased volume and were more densely arranged compared to those under the white light control,resulting in significantly improved agronomic traits and enhanced photosynthetic rates.These results suggest that high red light irradiation can be used in production to reduce mottling and yellowing in seedlings of the new line Hc400,while maintaining the desirable traits of the conventional cv.K326.关键词
烤烟/烟苗/品系Hc400/生理特性/光质/光合特性Key words
Flue-cured tobacco/Tobacco seedling/Line Hc400/Physiological characteristic/Light quality/Photosynthetic trait分类
农业科学引用本文复制引用
梁靖淞,王誉雯,黎娟,王东,彭宇,何鑫玺,熊坤龙,李军辉,彭光爵,秦天,陈伟浩..不同光质组合缓解烤烟新品系Hc400烟苗叶片花斑黄化的效果[J].烟草科技,2025,58(6):19-27,9.基金项目
湖南中烟工业有限责任公司科研项目"高钾雪茄型K326生产技术及其配方可用性研究—养分特性与抗性评价"(KY2022YC0005). （KY2022YC0005）
