烟草科技2025，Vol.58Issue(6)：28-37,10.DOI:10.16135/j.issn1002-0861.2025.0140
普通烟草长叶柄突变基因的图位克隆与候选基因分析
Map-based cloning and candidate analysis of mutant genes controlling long petiole in Nicotiana tabacum
摘要
Abstract
To understand the molecular mechanism underlying the establishment of leaf polarity,phenotypic analysis was performed on two long petiole mutants,M48 and M50,obtained by EMS(ethyl methanesulfonate)mutagenesis from tobacco(Nicotiana tabacum L.).Map-based cloning and comparative genomics strategies were used to isolate the mutant genes,followed by bioinformatic analysis of the candidate genes.The results showed that the long petioles of M48 and M50 were caused by the exposure of the leaf midvein,resulting from the degradation of the leaf blade from the base to the tip with different degrees.The mutant genes,Nta14g05160.1 and Nta13g05820.1,corresponding to M48 and M50,respectively,were identified as Arabidopsis REVOLUTA orthologs involved in the establishment of adaxial-abaxial polarity.Sequence similarity indicated that Nta14g05160.1 and Nta13g05820.1 originated from Nicotiana tomentosiformis and Nicotiana sylvestris,the diploid ancestors of Nicotiana tabacum L.,respectively.The mutations occurred at the microRNA165(miR165)binding site within the START domain coding sequence,suggesting that these mutations probably prevent microRNA-mediated degradation of mRNAs,resulting in increased gene transcript levels and ultimately the gain-of-function dominant mutant phenotypes.关键词
普通烟草/长叶柄/叶极性/图位克隆/REVOLUTA基因/小RNA/显性突变Key words
Nicotiana tabacum/Long petiole/Leaf polarity/Map-based cloning/REVOLUTA gene/MicroRNA/Dominant mutation分类
生物学引用本文复制引用
田震,刘贯山,吴新儒,赵勇,孙治峰,殷瑜东,王宝剑,刘西金,谢贺,张瑞,李雨,刘文涛..普通烟草长叶柄突变基因的图位克隆与候选基因分析[J].烟草科技,2025,58(6):28-37,10.基金项目
中国烟草总公司"揭榜挂帅"项目"中烟特香301品种品质保障技术优化及在中高档卷烟中的应用研究"(110202103015) （110202103015）
江苏中烟工业有限责任公司对外合作科技项目"中烟特香301新品种烟叶品质保障技术及应用研究"(H202110). （H202110）
