烟草科技2025，Vol.58Issue(6)：38-44,7.DOI:10.16135/j.issn1002-0861.2024.0938
丁香酚对尖孢镰刀菌的抑制效果及转录组分析
Inhibitory effect of eugenol on Fusarium oxysporum and transcriptome analysis
摘要
Abstract
To clarify the inhibitory effect and molecular mechanism of eugenol on Fusarium oxysporum(the pathogen of tobacco fusarium root rot disease),the inhibition rate of eugenol on mycelial growth of F.oxysporum was determined by the mycelial growth rate method.The inhibition rate of eugenol on spore germination of F.oxysporum was determined by the spore germination test.The effect of eugenol on the membrane integrity of F.oxysporum was analyzed by propidium iodide staining,conductivity and extracellular protein content determination.The molecular mechanism of eugenol inhibition of F.oxysporum was analyzed by transcriptome sequencing.The results showed that the inhibition rates of eugenol on the mycelial growth of F.oxysporum were 42.41%,53.96%,92.47%and 99.82%,respectively,after 72 h of culture on PDA medium with eugenol concentrations of 62.5,125.0,250.0 and 500.0 μg/mL.When eugenol was added to the spore suspension of F.oxysporum at concentrations of 62.5,125.0,250.0 and 500.0 μg/mL,the inhibition rates of eugenol on spore germination of F.oxysporum were 16.80%,24.34%,58.98%and 81.50%,respectively.According to propidium iodide staining,the membrane integrity of F.oxysporum treated with eugenol(250 μg/mL)was damaged.After treatment with eugenol(250 μ g/mL)for 12 h,the extracellular conductivity of F.oxysporum increased from 0.89 to 1.26 mS/cm.After treatment with eugenol(250 μg/mL)for 8 h,the soluble protein content increased from 0.236 7 to 0.911 5 mg/mL.Transcriptome sequencing analysis showed that there were 2 548 genes with significant differences between eugenol(250 μg/mL)treated and control 3 treatment.Among them,1 395 genes were up-regulated and 1 153 genes were down-regulated.GO functional classification analysis showed that the differentially expressed genes were mainly enriched in signal transduction,proteasome complex and nucleotide binding components.KEGG functional enrichment showed that the differentially expressed genes were mainly enriched in endocytosis,oxidative phosphorylation and purine metabolism.关键词
烟草/镰刀菌根腐病/尖孢镰刀菌/丁香酚/抑制效果/转录组分析Key words
Tobacco/Fusarium root rot disease/Fusarium oxysporum/Eugenol/Inhibitory effect/Transcriptome analysis分类
植物保护学引用本文复制引用
彭姝尧,张洪郝,许汝冰,常晓涵,周子豪,杨勇,向海波,黎妍妍..丁香酚对尖孢镰刀菌的抑制效果及转录组分析[J].烟草科技,2025,58(6):38-44,7.基金项目
中国烟草总公司重点科技项目"基于微生物组的烟草根际生物屏障抗病机理及应用研究"(110202101047[LS-07])、"烟草生防菌精准鉴定、重要性状解析及资源库建设"(110202201019[LS-03]) （110202101047[LS-07]）
湖北省烟草公司重点科技项目"雪茄烟主要病虫害灾变规律调查及防控技术研究与应用"(027Y2022-023). （027Y2022-023）
