针刺研究2025，Vol.50Issue(6)：633-640,8.DOI:10.13702/j.1000-0607.20240070
基于JAK2/STAT3通路探讨"夹脊"电针治疗脊髓损伤大鼠的神经元凋亡作用机制
Exploration of the mechanism of electroacupuncture at"Jiaji"(EX-B2)in treating neuronal apoptosis in rats with spinal cord injury based on the JAK2/STAT3 pathway
摘要
Abstract
Objective To observe the effect of electroacupuncture(EA)at"Jiaji"(EX-B2)on inhibiting neuronal apoptosis in rats with spinal cord injury based on the Janus tyrosine kinase(JAK)/signal transducer and activator of transcription(STAT)signaling pathway,so as to explore the underlying mechanism of EA in the treatment of spinal cord injury.Methods A total of 36 SD rats were randomly divided into sham operation group,spinal cord injury group,and Jiaji EA group,with 12 rats in each group.The spinal cord injury model was established using Allen's method.The Jiaji EA group was treated with EA at EX-B2(once a day,30 min each time,for a total of 14 d).After the treatment,the Basso Beattie Bresnahan(BBB)score was used to evaluate the hindlimb motor function of rats in each group.HE staining was used to observe the morphological changes of spinal cord tissues.TUNEL staining was used to observe the pathological changes and neuronal apoptosis rate of the spinal cord.Western blot was used to detect the relative expression levels of phosphorylated(p)-JAK2,p-STAT3,B-cell lymphoma-2(Bcl-2),Bcl-2 associated X protein(Bax),and Caspase-3 proteins in the spinal cord.Results Compared with the sham operation group at the same time point,the BBB score decreased(P<0.01),the spinal cord tissue structure in the injury area was disordered,the cell body shrank,and the neuronal apoptosis rate increased(P<0.01),the expression levels of p-JAK2,p-STAT3,Bax,and caspase-3 proteins increased(P<0.01)while the expression level of Bcl-2 protein decreased(P<0.01)in rats of the spinal cord injury group.Compared with the spinal cord injury group,the BBB score increased on the 7th and 14th day of intervention(P<0.01),the spinal cord tissue structure tended to be normal,the degree of neuronal damage was reduced,and the apoptosis rate decreased(P<0.01),the expression levels of p-JAK2,p-STAT3,Bax,and caspase-3 proteins decreased(P<0.01,P<0.05),while the expression level of Bcl-2 protein increased(P<0.05)in rats of the Jiaji EA group.Conclusion EA at EX-B2 can improve the motor function of rats with spinal cord injury,up-regulate the expressions of anti-apoptotic factors,down-regulate the expressions of pro-apoptotic factors,and reduce neuronal apoptosis in spinal cord injury.The mechanism may be related to the inhibition of the activation of the JAK2/STAT3 signaling pathway.关键词
脊髓损伤/夹脊/电针/细胞凋亡/Janus酪氨酸蛋白激酶(JAK)/信号转导及转录激活因子(STAT)信号通路Key words
Spinal cord injury/Jiaji(EX-B2)/Electroacupuncture/Apoptosis/Janus tyrosine kinase(JAK)/signal transducer and activator of transcription(STAT)signaling pathway引用本文复制引用
蒋焕莹,尹洪娜,张东旭,孙忠人,焦雪峰,周明园,黄飞,洛子茹,孙美琦,开心..基于JAK2/STAT3通路探讨"夹脊"电针治疗脊髓损伤大鼠的神经元凋亡作用机制[J].针刺研究,2025,50(6):633-640,8.基金项目
国家自然科学基金面上项目(No.81873378) （No.81873378）
