针刺研究2025，Vol.50Issue(6)：658-665,8.DOI:10.13702/j.1000-0607.20240157
热敏灸调节变应性鼻炎大鼠Th17/Treg免疫失衡的机制研究
Mechanism of thermo-sensitive moxibustion intervention in regulating Th17/Treg immune imbalance in the rat model of allergic rhinitis
摘要
Abstract
Objective To investigate the effects of thermo-sensitive moxibustion on helper T cells(Th)17/regulatory T cells(Treg)immune imbalance and related cytokines in the rat model of allergic rhinitis(AR).Methods Thirty 8-week-old SD rats were randomly divided into a blank group(n=6),a model group(n=6),a western medicine group(n=6),and a moxibustion group(n=12).AR model was established by intraperitoneal injection of ovalbumin and aluminium hydroxide suspension for 14 d followed by intranasal administration of 20 μL of 12%ovalbumin for 10 days.After successful modeling,rats in the western medicine group received 8 μL of fluticasone propionate intranasally for 21 days;the moxibustion group was divided into a thermo-sensitive moxibustion group(n=4)and a traditional moxibustion group(n=4)based on whether the increase in tail temperature detected by an infrared thermal imager was greater than 1 °C.Both moxibustion groups received moxibustion on"Feishu"(BL13)for 40 minutes,for 21 days.After the intervention,HE staining was used to observe the morphological changes of the nasal mucosa;ELISA was performed to measure serum interleukin(IL)-17A,IL-10,and transforming growth factor(TGF)-β1 contents;and immunohistochemical staining was used to observe the expression of IL-17A,retinoid-related orphan nuclear receptor γt(RORγt),IL-10,TGF-β1,and forkheadbox protein 3(FOXP3)in the nasal mucosa;flow cytometry was used to detect the percentage of Th17 and Treg in peripheral blood.Results Compared with the blank group,rats in the model group displayed swelling and deformation of the nasal mucosa,significant eosinophil infiltration,and increased serum IL-17A contents along with increased expression of IL-17A and RORγt in the nasal mucosa,as well as a significant rise in peripheral blood Th17 percentage(P<0.001,P<0.05);while serum IL-10 and TGF-β1 contents,along with FOXP3,IL-10,and TGF-β1 expression in the nasal mucosa and peripheral blood Treg percentage were significantly reduced(P<0.05,P<0.001).Compared with the model group,no significant thickening or eosinophil infiltration was found in the nasal mucosa of the western medicine,traditional moxibustion,or thermo-sensitive moxibustion groups,with improvements of the above indicators observed in all groups to varying extents(P<0.001,P<0.01,P<0.05).Compared with the thermo-sensitive moxibustion group,the western medicine group showed decreased expression of FOXP3 in the nasal mucosa(P<0.05),while serum IL-17A contents and nasal mucosal TGF-β1,RORγt,and IL-17A expressions were increased(P<0.001,P<0.01,P<0.05),with an increase in peripheral blood Th17 percentage(P<0.01)and a decrease in Treg cells(P<0.01);Traditional moxibustion group showed significantly reduced levels of serum IL-10 content and IL-10 expression in the nasal mucosa(P<0.001),along with higher expression of RORγt and IL-17A(P<0.001,P<0.01).Compared with the traditional moxibustion group and westernmedicine group,the thermo-sensitive moxibustion group had milder nasal mucosa swelling,less eosinophil infiltration.Conclusion Thermo-sensitive moxibustion may alleviate allergic rhinitis by regulating Th17/Treg immune balance and related cytokines.关键词
热敏灸/变应性鼻炎/辅助性T细胞(Th)17/调节性T细胞(Treg)免疫失衡/机制研究Key words
Thermo-sensitive moxibustion/Allergic rhinitis/Th17/Treg immune imbalance/Mechanism study引用本文复制引用
管灵聪,熊俊,郭安吉,李逸成,崔淑君,罗菡玲..热敏灸调节变应性鼻炎大鼠Th17/Treg免疫失衡的机制研究[J].针刺研究,2025,50(6):658-665,8.基金项目
国家自然科学基金项目(No.82060893) （No.82060893）
江西省教育厅科技计划项目(No.GJJ190629) （No.GJJ190629）
江西省博士后科研项目择优资助项目(No.2020KY08) （No.2020KY08）
