新发传染病电子杂志2025，Vol.10Issue(2)：24-28,5.DOI:10.19871/j.cnki.xfcrbzz.2025.02.005
结核分枝杆菌RNA检测在耐药肺结核患者疗效监测中的临床价值研究
Clinical value of Mycobacterium tuberculosis RNA detection in efficacy monitoring of drug-resistant pulmonary tuberculosis patients
摘要
Abstract
Objective The positive detection rate of Mycobacterium tuberculosis ribonucleic acid(TB-RNA)detection was compared with that of traditional methods,and the clinical value of Mycobacterium tuberculosis ribonucleic acid(TB-RNA)detection in monitoring the efficacy of drug-resistant pulmonary tuberculosis patients was explored.Method A total of 117 patients with drug-resistant pulmonary tuberculosis who were hospitalized in the tuberculosis Department of Guangxi Chest Hospital from November 2022 to May 2024 were selected.The positive detection rates of TB-RNA,smear and solid culture were compared at baseline and from 1 to 6 months after treatment.Kappa test was used to evaluate the consistency of TB-RNA and smear results with solid culture results.Result Using clinical diagnosis as a reference standard,the positive detection rate of sputum specimens for TB-RNA testing was higher than that of solid cultures and smears at baseline and during the treatment period of 1-6 months.Among them,there was a statistically significant difference between the baseline and the treatment period of 1 month,2 month,4 month and 5 month(P＜0.05).At baseline and during 1,2 and 4 months of treatment,the difference was statistically significant compared with solid culture(P＜0.05).Using the results of solid culture and strain identification as reference standards,the sensitivity and negative predictive value of TB-RNA were higher than that of smear at baseline and during 1,2 and 4 months of treatment.Kappa concordance between TB-RNA and solid culture results was higher than smear.Conclusion TB-RNA has good efficacy in monitoring the efficacy of drug-resistant pulmonary tuberculosis.At present,although it cannot completely replace traditional methods,TB-RNA is more suitable for long-term efficacy monitoring of drug-resistant pulmonary tuberculosis.关键词
结核分枝杆菌核糖核酸/耐药肺结核/疗效监测/Kappa分析/临床价值Key words
Mycobacterium tuberculosis ribonucleic acid/Drug-resistant pulmonary tuberculosis/Efficacy detection/Kappa analysis/Clinical value分类
临床医学引用本文复制引用
周游,何希,黄丽花,罗兰波,胡敏,陈桂彬,刘爱梅,黄启文,杨小兵..结核分枝杆菌RNA检测在耐药肺结核患者疗效监测中的临床价值研究[J].新发传染病电子杂志,2025,10(2):24-28,5.基金项目
1.广西医疗卫生适宜技术开发与推广应用项目(S2021031) （S2021031）
2.广西科技重大专项(桂科AA22096027) （桂科AA22096027）
