湖北医药学院学报2025，Vol.44Issue(3)：260-264,5.DOI:10.13819/j.issn.2096-708X.2025.03.002
拟南芥CERK1F322位点影响先天免疫的研究
Studies of the CERK1 F322 Locus Affecting Innate Immunite Response in Arabidopsis
摘要
Abstract
Objective To study the function of conserved amino acids in the juxtamembrane domain of CERK1 in Arabidopsis thaliana in mediating chitin-induced immune responses.Methods Bioinformatics was employed to analyze the conservation of amino acids in the LysM receptor family of A.thaliana.Mutants of CERK1 and conserved amino acid residues in the in-tracellular juxtamembrane domain were constructed and expressed transiently in Nicotiana benthamiana to verify their im-mune functions.Using Arabidopsis transformation technology,the roles of these conserved amino acid residues were investi-gated in mediating chitin-induced immune responses through quantitative fluorescence assay and Western blot in conserved amino acid mutations in juxtamembrane domain of CERK1.Results Amino acid sequences in the juxtamembrane domain of the LysM receptor kinase family of A.thaliana were analyzed,which identified three conserved amino acid sites in CERK1:T319,F322,and S325.The overexpression of CERK1F322A resulted in the disability of inducing hypersensitive response in N.benthamiana.In CERK1F322A transgenic lines,the transcriptions of WRKY33 and WRKY53 in response to chitin were sup-pressed,along with the phosphorylation modification of MAPK3/6.Conclusion The F322 locus is a critical amino acid site for CERK1 in transducing immune signals,highlighting its significant role in understanding the immune regulation by the juxtamembrane domain of CERK1 and the transduction of immune signals.关键词
CERK1/近膜域/先天免疫反应/几丁质受体Key words
CERK1/Juxtamembrane domain/Innate immune response/Chitin receptor引用本文复制引用
徐道修,王施祺,江振文,黄聪聪..拟南芥CERK1F322位点影响先天免疫的研究[J].湖北医药学院学报,2025,44(3):260-264,5.基金项目
湖北省大学生创新创业训练项目(202413249004) （202413249004）
湖北医药学院人才启动金项目(2020QDJZR024) （2020QDJZR024）
