木犀草素调控Notch信号通路抑制非小细胞肺癌细胞恶性进展OA

Objective To investigate the role of luteolin in inhibiting malignant proliferation of non-small cell lung cancer(NSCLC)cells and to verify whether this inhibitory effect is mediated by regulating the Notch signaling pathway.Methods In this experiment,human NSCLC cells were treated with luteolin.Cell proliferation activity was quantitatively evaluated u-sing the CCK-8 assay kit,while cell apoptosis was monitored using Hoechst 33258 staining and flow cytometry.Western blot was performed to detect the expressions of apoptosis-related proteins and those in the Notch signaling pathway.Subse-quently,a Notch pathway activator(Jagged1)was administered alongside luteolin treatment to assess the impact of luteolin on cellular biological behavior via the Notch signaling pathway.Results After 24 and 48 h of treatment with luteolin,cell viability was reduced in A549,NCI-H292,NCI-H596,SK-MES-1,and NCI-H460 cells,with the effects becoming more pronounced as the concentration of luteolin increased and the intervention time extended.A549 cells were the most sensitive and were chosen for further study;they were treated with luteolin at three concentrations(20,40,80 μmol/L)for 24 h.Hoechst 33258 staining and protein expression analysis indicated that luteolin promoted the apoptosis in A549 cells by up-regulating caspase-3,caspase-9,and Bax while down-regulating Bcl-2.Additionally,luteolin inhibited the pro-teins in the Notch signaling pathway Notch1 and HES1,and the inhibition was more significant under higher concentration.Following the addition of Jagged1,the inhibitory effect of luteolin on the Notch signaling pathway was diminished,which led to an increase in A549 cell survival,a decrease in apoptosis,down-regulations of caspase-3,caspase-9,and Bax,and an increase in Bcl-2.Conclusion Luteolin can inhibit the malignant progression of NSCLC cells by regulating the Notch signaling pathway,which hinders cell proliferation and promotes apoptosis.