综合分析RAB3B在肝细胞癌中的表达及其与糖酵解的关系OA

Objective To analyze the expression pattern of RAB3B in hepatocellular carcinoma(LIHC)and its correlation with diagnosis,prognosis,and tumor cell glycolysis using bioinformatics and immunohistochemical(IHC)staining.Meth-ods The data from TCGA and GEO databases were utilized to explore the role of RAB3B in LIHC through differential gene screening,correlation analysis,diagnostic efficacy assessment,prognostic analysis,functional enrichment analysis,and glycolysis correlation analysis.The expression differences of RAB3B were verified by IHC staining.Results The expression of RAB3B was higher in tumor samples than that in normal samples(P＜0.05)and it displayed high diagnostic efficacy(TCGA LIHC;AUC:0.878,95%CI=0.841～0.916).The expression of RAB3B was closely correlated with overall surviv-al and relapse-free survival of patients(P＜0.05).IHC staining confirmed that RAB3B in tumor samples was higher than that in adjacent normal tissues(P＜0.05).Functional enrichment analysis indicated that RAB3B was associated with 329 genes involved in glycoprotein biosynthesis and endoplasmic reticulum protein processing.Moreover,RAB3B was positively correlated with the mRNA expression of the glycolysis key gene GPI(P＜0.05).Conclusion The high expression of RAB3B is closely related to the progression,prognosis,and glycolysis of LIHC patients,suggesting it may serve as a biomarker for predicting the prognosis and therapeutic target in LIHC.