铁皮石斛-牻牛儿基-牻牛儿基焦磷酸合成酶基因的生物信息学分析OA

Objective To analyze the GGPS gene family in Dendrobium officinale,aiming to understand its coding informa-tion,function,and homology relationships.Methods Bioinformatics was employed to assess the physicochemical properties of the amino acids of three genes in the GGPS gene family,predict protein secondary and tertiary structures,perform amino acid sequence alignment,and construct phylogenetic trees.Comprehensive insights into the physiological and biochemical roles of the GGPS gene family members were gathered.Results The GGPS gene consisted of three subunits,namely,DoG-GPPS,DoGGR,and DoGGPPS1.These three proteins were characterized as unstable and did not contain signal peptide sites.DoGGR and DoGGPPS1 had glycosylation sites,and none of the three proteins possessed transmembrane structures,with secondary structures predominantly consisting of α-helices.Conclusion The bioinformatics analysis of the GGPS gene is conducive to the cloning and further functional research of this gene.