中国临床药理学杂志2025，Vol.41Issue(8)：1108-1113,6.DOI:10.13699/j.cnki.1001-6821.2025.08.011
基于方剂反证策略的多囊卵巢综合征肾虚血瘀证大鼠模型构建与评价
Construction and evaluation of polycystic ovary syndrome rat model with kidney deficiency and blood stasis based on prescription counter-evidence strategy
摘要
Abstract
Objective To establish an animal model of polycystic ovary syndrome(PCOS)characterized by kidney deficiency and blood stasis,and to evaluate its efficacy.Methods SD rats were randomly divided into normal group,PC OS group,model group and formula counter-evidence group,with 10 rats in each group.The normal group was given 1 mg·kg-1 1％sodium carboxymethyl cellulose daily by intragastric administration;PCOS group was given 1 mg·kg-1 letrozole daily by intragastric administration;on the basis of PCOS group,the model group was injected subcutaneously with 0.6 mg·kg-1 epinephrine hydrochloride solution daily for 2 weeks from day 21.On the basis of the model group,the formula counter-evidence group was given 12.51 g·kg-1 Zhu's Tiaojing decoction daily for 2 weeks from the 21st day.After 35 days of intervention,the indexes of ovarian index,testosterone,vitamin D and whole blood low shear rate were compared.Results The ovarian indexes of normal,PCOS,model and formula counter-evidence groups were(6.39±0.58)× 10-4,(5.32±0.71)× 10-4,(5.05±0.29)× 10-4 and(8.17±0.65)× 10-4;testosterone concentrations were(7.10±0.97),(9.22±0.43),(9.06±1.21)and(7.73±0.76)nmol·L-1;vitamin D levels were(1 850.98±227.95),(986.10±152.50),(1 220.67±64.35)and(1 598.20±294.75)pg·mL-1;whole blood low shear rates were(13.00±1.79),(13.37±0.54),(16.38±1.51)and(11.43±2.77)mPa·s,respectively.The above indexes in the model group were statistically significant compared with the formula counter-evidence group and the normal group(all P＜0.05).Conclusion Letrozole combined with epinephrine hydrochloride can stably replicate the PCOS rat model with kidney deficiency and blood stasis syndrome.关键词
来曲唑/盐酸肾上腺素/多囊卵巢综合征/肾虚血瘀/动物模型Key words
letrozole/epinephrine hydrochloride/polycystic ovary syndrome/kidney deficiency and blood stasis/animal model分类
中医学引用本文复制引用
李泉洋,郝亚芳,吴国泰,杜丽东,王瑞琼..基于方剂反证策略的多囊卵巢综合征肾虚血瘀证大鼠模型构建与评价[J].中国临床药理学杂志,2025,41(8):1108-1113,6.基金项目
国家自然科学基金地区基金资助项目(81660653) （81660653）
甘肃省自然科学基金资助项目(22JR11RA588) （22JR11RA588）
甘肃省创新基地和人才计划基金资助项目(21JR11RA191) （21JR11RA191）
甘肃省青年博士基金资助项目(2021QB-074) （2021QB-074）
甘肃省人民医院院内科研基金资助项目(21GSSYB-37) （21GSSYB-37）
