中国临床药理学杂志2025，Vol.41Issue(8)：1114-1118,5.DOI:10.13699/j.cnki.1001-6821.2025.08.012
基于脂代谢通路探索白藜芦醇对糖尿病大鼠肾的保护作用
Protective effects of resveratrol on kidney of diabetic rats based on lipid metabolism pathway
摘要
Abstract
Objective To observe the protective effect of resveratrol on kidney of diabetic rats through lipid metabolism pathway.Methods The SD rats were randomly divided into control group,model group and experimental-L,-M,-H groups.The control group was fed with normal feed,while the other four groups were all based on high-fat and high-sugar feed feeding+intraperitoneal injection of streptozotocin to establish diabetic rat models.After successful modeling,the control group and the model group were given the same amount of normal saline,while the experimental-L,-M,-H groups were given 5,25,50 mg·kg-1 concentration of resveratrol once a day,and were given continuous gavage for 10 weeks.Kidney function levels[cystatin C(Cys-C),serum creatinine(SCr),blood urea nitrogen(BUN)]were determined by enzyme-linked immunosorbent assay.The expressions of peroxissome proliferator-activated receptor γ(PPARγ),fatty acid-binding protein(FABP1),peroxisomal acyl-coenzyme A oxidase 1(ACOX)2 and ACOX1 were detected by Western blot.Results The Cys-C levels of experimental-M,-H groups,control group,model group were(1.85±0.15),(1.39±0.11),(0.78±0.11)and(2.42±0.32)mg·L-1;SCr levels were(55.74±7.02),(48.15±6.97),(32.41±4.22)and(68.15±8.48)μmol·L-1;BUN levels were(10.37±1.02),(9.41±0.87),(7.12±1.12)and(14.48±2.45)mmol·L-1;the relative expression levels of PPARγ protein were 0.74±0.08,0.92±0.10,1.22±0.15 and 0.39±0.05;the relative expression levels of FABP1 protein were 0.64±0.08,0.81±0.07,0.98±0.09 and 0.30±0.05;the relative expression levels of ACOX2 protein were 0.59±0.06,0.72±0.07,0.85±0.13 and 0.27±0.05;the relative expression levels of ACOX1 protein were 0.58±0.06,0.67±0.05,0.78±0.06 and 0.24±0.03.The differences of above indexes were statistically significant between the experimental-M,-H groups and control group and the model group(all P＜0.05).Conclusion Resveratrol may play a protective role on kidney of diabetic rats by regulating lipid metabolism pathway.关键词
白藜芦醇/脂代谢通路/糖尿病/肾保护Key words
resveratrol/lipid metabolism pathway/diabetes mellitus/kidney protection分类
中医学引用本文复制引用
张惠娜,于敏,谷巍,侯丽萍,程黎蓉..基于脂代谢通路探索白藜芦醇对糖尿病大鼠肾的保护作用[J].中国临床药理学杂志,2025,41(8):1114-1118,5.基金项目
河北省医学科学研究课题计划基金资助项目(20242094) （20242094）
