中国临床药理学杂志2025，Vol.41Issue(8)：1126-1131,6.DOI:10.13699/j.cnki.1001-6821.2025.08.014
淫羊藿苷对复发性流产小鼠免疫调节作用及TLR4信号通路的影响
Effects of Icariin on immune regulation and TLR4 signaling pathway in mice with recurrent abortion
摘要
Abstract
Objective To investigate the protective effect of icariin on recurrent spontaneous abortion(RSA)mice and its potential mechanism.Methods Female CBA/J mice were randomly divided into control group(mated with male B ALB/c mice),model group(modeling mating with male DBA/2 mice),experimental-L group(after modeling,25.00 mg·kg-1 icariin was given by intragastric administration),experimental-H group(50.00 mg·kg-1 icariin was given by intragastric administration after modeling),positive group(2.6 mg·kg-1 desdrogesterone was given by intragastream after modeling),uterine tissue was collected after 14 days of treatment for follow-up experiments.Embryo absorption rate was observed and recorded.The levels of interleukin(IL)-4 and interferon γ(IFN-γ)were detected by enzyme linked immunosorbent assay(ELISA).The mRNA expressions of Foxp3 and Toll-like receptor 4(TLR4)were detected by real-time fluorescence quantitative polymerase chain reaction(RT-qPCR).Western blot assay was used to detect the expression of TLR4 and phosphorylated nuclear transcription factor-κB(p-NF-κB)protein.Results The absorption rates of mouse embryos in control group,model group,experimental-L group,experimental-H group and positive group were 7.37％(7 cases/95 cases),37.33％(28 cases/75 cases),20.93％(18 cases/86 cases),13.79％(12 cases/87 cases)and 12.22％(11 cases/90 cases),respectively;IL-4 expression levels were(33.84±5.71),(10.71±2.34),(16.86±1.13),(26.91±3.04)and(19.56±1.79)pg·mL-1,respectively;the expression levels of IFN-γ were(25.97±1.40),(63.38±5.39),(47.23±6.78)(34.80±4.08)and(29.61±3.25)pg·mL-1,respectively;the relative expression levels of Foxp3 mRNA were 1.00±0.07,0.57±0.05,0.72±0.06,0.90±0.05 and 0.66±0.06,respectively;the relative expression levels of TLR4 mRNA were 1.00±0.10,2.32±0.17,1.78±0.14,1.40±0.08 and 2.05±0.26,respectively;the relative expression levels of TLR4 protein were 0.31±0.05,0.90±0.11,0.68±0.05,0.55±0.05 and 0.79±0.10,respectively;the relative expression levels of p-NF-κB protein were 0.27±0.02,0.82±0.13,0.66±0.04,0.42±0.05 and 0.75±0.09,respectively.The above indicators in the model group compared with the control group,the experimental-L and experimental-H groups were compared with the model group,and the differences were statistically significant(all P＜0.05).Conclusion Icariin may play a protective role in improving RSA immune response by inhibiting TLR4/NF-κB pathway.关键词
淫羊藿苷/复发性自然流产/免疫应答/辅助性T细胞1/2平衡/Toll样受体4信号通路Key words
icariin/recurrent spontaneous abortion/immune response/T helper cell 1/2 balance/Toll-like receptor 4 signaling pathway分类
中医学引用本文复制引用
李金芬,朱俊江,刘媛媛,罗吉,梁旭晖,肖建英..淫羊藿苷对复发性流产小鼠免疫调节作用及TLR4信号通路的影响[J].中国临床药理学杂志,2025,41(8):1126-1131,6.基金项目
江西省卫生健康委员会科技计划基金资助项目(20202078) （20202078）
