中国临床药理学杂志2025，Vol.41Issue(8)：1132-1137,6.DOI:10.13699/j.cnki.1001-6821.2025.08.015
川芎挥发油治疗原发性痛经的作用研究
Research on effects of essential oil of Ligusticum chuanxiong on primary dysmenorrhea
摘要
Abstract
Objective To explore the effects of essential oil of Ligusticum chuanxiong(LCEO)on primary dysmenorrhea(PD)and preliminarily its mechanism of action.Methods Forty-eight female non-pregnant KM mice were randomly divided into blank group,model group,positive control group and experimental-L,-M,-H groups.Except for the blank group,the remaining groups were gavaged with estradiol valerate to establish the PD mouse model.Starting from the 7th day,the blank group and the model group were gavaged with equal volumes of distilled water in the morning,the positive control group was administered 120 mg·kg-1 of ibuprofen solution by gastric lavage,while the low,medium,and high dose experimental groups were gavaged with 22,44 and 88 mg·kg-1 of LCEO,respectively,for 5 consecutive days.Enzyme-linked immunosorbent assay(ELISA)was used to measure the levels of oxytocin(OT)and prostaglandin F2α(PGF2α)in mice uterine.Using the oxytocin-induced rat isolated uterine contraction model,the study explored the mechanism of LCEO in relaxing uterine smooth muscle through preincubation with different blocking agents(Indomethacin,phentolamine mesylate,propranolol hydrochloride,atropine sulfate,diphenhydramine hydrochloride,ranitidine hydrochloride and nifedipine).Results The OT contents in the blank group,model group,positive control group and experimental-L,-M,-H groups were(3.57±0.81),(5.54±0.60),(4.63±0.75),(4.15±0.76),(4.04±1.19)and(3.67±0.66)pg·mg-1;the PGF2α contents were(1.22±0.38),(2.53±0.45),(1.84±0.52),(1.30±0.31),(1.21±0.35)and(0.48±0.15)pg·mg-1,respectively.The above indexes in the model group were compared with the experimental-L,-M,-H and blank,positive control groups,there were statistically significant differences in all comparisons(P＜0.01,P＜0.05).After preincubation with indomethacin,phentolamine mesylate,and atropine sulfate,the relaxant effect on uterine smooth muscle of LCEO was significantly weakened.After preincubation with propranolol hydrochloride,diphenhydramine hydrochloride,ranitidine hydrochloride and nifedipine,the relaxant effect on uterine smooth muscle of LCEO did not show any significant changes.Conclusion LCEO has a significant effect on primary dysmenorrhea,and its mechanism may be related to inhibiting cyclooxygenase and blocking receptors α and M.关键词
川芎/挥发油/原发性痛经/子宫平滑肌/作用机制Key words
Ligusticum chuanxiong Hort./essential oil/primary dysmenorrhea/uterine smooth muscle/mechanism of action分类
中医学引用本文复制引用
马凤至,熊亮,冯芮,刘娟,周勤梅,戴鸥..川芎挥发油治疗原发性痛经的作用研究[J].中国临床药理学杂志,2025,41(8):1132-1137,6.基金项目
国家自然科学基金面上基金资助项目(82374152) （82374152）
国家自然科学基金优秀青年基金资助项目(82022072) （82022072）
四川省自然科学基金资助项目(2024NSFSC1830) （2024NSFSC1830）
四川省科技创新人才计划基金资助项目(2023JDRC0041) （2023JDRC0041）
