中国临床药理学杂志2025，Vol.41Issue(8)：1138-1143,6.DOI:10.13699/j.cnki.1001-6821.2025.08.016
水菖蒲通过抑制MAPK/NF-κB信号通路对大鼠放射性皮肤损伤创面愈合的研究
Research of Acorus calamus L.inhibiting MAPK/NF-κB signaling pathway on healing of radioactive skin injury in rats
摘要
Abstract
Objective To investigate the effect of Acorus calamus L.on wound healing of radioactive skin injury in rats based on the mitogen-activated protein kinase(MAPK)/nuclear factorκB(NF-κB)signaling pathway.Methods A rat model of radioactive skin injury was induced by the radionuclide irradiation,the successful model rats were randomly divided into the model group,the control group,and the experimental-H,-M,-L groups with 12 rats in each group,and another 12 normal rats were taken as the normal group.Experimental-H,-M,-L goups was applied with Acorus calamus L.600,400,200 g·kg-1,respectively.Control group was applied with triethanolamine cream 400 g·kg-1 on rat wounds;normal and model groups were treated with 0.9％NaCl.Six groups were treated once a day for 45 days.The healing rate of wounds was compared among the six groups.The levels of interleukin(IL)-1 β,IL-6 and tumor necrosis factor(TNF)-α in serum and hydroxyproline(HYP)in wound tissue were measured by enzyme linked immunosorbent assay.The protein levels of IL-1 β,IL-6,TNF-α,mitogen-activated protein kinase(p38)and nuclear factor κB p65 subunit(p65)were detected by Western blot.Results The wound healing rates of experimental-H,-M,-L groups,control group and model group were(70.24±3.17)％,(62.41±4.52)％,(49.89±4.61)％,(50.35±2.44)％and(29.42±4.23)％,respectively.The levels of IL-1 β in experimental-H,-M,-L groups,control group,model group and normal group were(41.22±2.98),(52.35±1.69),(61.32±2.74),(45.26±3.12),(79.24±5.64)and(28.85±2.11)pg·L-1;the levels of IL-6 were(25.68±1.65),(32.55±2.64),(51.23±2.34),(32.32±1.56),(61.12±2.35)and(18.88±1.12)pg·L-1;the levels of TNF-α were(62.38±5.66),(73.56±4.52),(88.63±5.68),(68.33±4.32),(112.48±6.52)and(55.21±3.89)pg·L-1;the levels of HYPs of(3.77±0.41),(3.51±0.26),(2.98±0.39),(3.62±0.37),(2.21±0.48)and(4.22±0.55)μg·mg-1;the relative expression levels of IL-1 β were 0.31±0.04,0.43±0.07,0.60±0.03,0.51±0.04,0.69±0.06 and 0.11±0.07;the relative expression levels of IL-6 were 0.50±0.03,0.60±0.04,0.69±0.04,0.62±0.07,0.83±0.08 and 0.28±0.06;the relative expression levels of TNF-α were 0.49±0.06,0.61±0.03,0.73±0.06,0.59±0.04,0.83±0.05 and 0.28±0.04;the phosphorylated p38/p38 ratios were 0.46±0.06,0.56±0.04,0.67±0.03,0.56±0.05,0.84±0.04 and 0.34±0.08;the phosphorylated p65/p65 ratios were 0.37±0.06,0.54±0.03,0.63±0.04,0.47±0.05,0.79±0.04,and 0.32±0.06,respectively.Compared with model group,the above indexes in the experimental-H,-M groups were statistically significant(all P＜0.05).Conclusion Acorus calamus L.can promote the healing of radioactive skin injury in rats,inhibit the inflammation level of the wound tissue,and promote collagen deposition,and its mechanism may be related to the regulation of MAPK/NF-κB pathway.关键词
水菖蒲/放射性皮肤损伤/丝裂原活化蛋白激酶/核因子κB信号通路Key words
Acorus calamus L./radiation skin reaction/mitogen-activated protein kinase/nuclear factor kappa-B signaling pathway分类
医药卫生引用本文复制引用
武晓丹,童亚楠,战莹,张文文,王治国,张国旭..水菖蒲通过抑制MAPK/NF-κB信号通路对大鼠放射性皮肤损伤创面愈合的研究[J].中国临床药理学杂志,2025,41(8):1138-1143,6.基金项目
国家自然科学基金青年基金资助项目(82102744) （82102744）
辽宁省民生科技计划联合计划基金资助项目(2022JH2/101500021) （2022JH2/101500021）
