中国临床药理学杂志2025，Vol.41Issue(8)：1157-1163,7.DOI:10.13699/j.cnki.1001-6821.2025.08.019
基于LC-MS/MS法分析栀子16种功效成分的小鼠组织分布
Analysis of tissue distribution of 16 functional components of Gardenia jasminoides Ellis based on LC-MS/MS in mice
摘要
Abstract
Objective To develop a liquid chromatography-tandem mass spectrometry(LC-MS/MS)method for quantifying 16 bioactive constituents of Gardenia jasminoides Eills in various murine tissues.Methods The ileum,colon,stomach,liver,kidney and brain tissues of mice were collected following intragastric administration of Gardenia jasminoides Ellis.Subsequently,the samples were processed by precipitating protein.The analysis was performed using the Waters CORTECS C18 column(4.6 mm × 150.0 mm,2.7 μm)with mobile phase gradient elution consisting of a methanol-water solution,and specnuezhenide was used as the internal standard;the flow rate was set at 0.4 mL·min-1 while maintaining a column temperature of 40 ℃;detection was performed in negative ion mode utilizing an electrospray ion source.Furthermore,the method was evaluated for its specificity,standard curve and lower limit of quantification,precision and recovery,stability and reproducibility.Additionally,the tissue distribution characteristics of the active components of Gardenia jasminoides Ellis in mice were investigated.Results This study established a method for the analysis of 16 bioactive constituents in Gardenia jasminoides Ellis within complex matrices.The linear range spanned from 0.98 to 6.00 × 104ng·mL-1,with a limit of quantitation ranging from 0.92 to 11.72 ng·mL-1,and the RSD values for intra-day and inter-day precision as well as recovery were all below 15％.Amongst the examined tissues,ileum tissue exhibited the highest levels of these active components,followed by gastric tissue.Iridoid components demonstrated extensive distribution across various tissues.The diterpenoid constituent crocetin was predominantly found in the liver and brain,while crocinⅡ showed higher distribution in the colon.Conclusion The method exhibits high specificity,excellent sensitivity,reproducibility,and stability.Moreover,the matrix effect can be controlled effectively,rendering it suitable for analyzing Gardenia jasminoides Ellis in complex matrices.The intestine is the major tissue for the distribution of Gardenia jasminoides Ellis,which may be an important part for its efficacy.Furthermore,the extensive pharmacological effects of Gardenia jasminoides Ellis may be related to the component tendency to different tissues.关键词
栀子/环烯醚萜类/二萜类/液相色谱-质谱联用仪/功效成分/组织分布Key words
Gardenia jasminoides Ellis/iridoids/diterpenoids/liquid chromatography-tandem mass spectrometry/active component/tissue distributio分类
医药卫生引用本文复制引用
秦佳琪,饶志,吕东霞,张帆,雒馨怡,魏玉辉..基于LC-MS/MS法分析栀子16种功效成分的小鼠组织分布[J].中国临床药理学杂志,2025,41(8):1157-1163,7.基金项目
国家自然科学基金资助项目(82174067,82460856) （82174067,82460856）
甘肃省卫生健康委员会中医药科研课题资助项目(GZKP-2O22-33) （GZKP-2O22-33）
甘肃省联合科研基金资助项目(23JRRA1492) （23JRRA1492）
兰州大学第一医院院内基金资助项目(ldyyyn2023-34) （ldyyyn2023-34）
