中国免疫学杂志2025，Vol.41Issue(6)：1344-1349,6.DOI:10.3969/j.issn.1000-484X.2025.06.015
ERMAP改善MOG特异性TCR转基因小鼠实验性自身免疫性脑脊髓炎
ERMAP ameliorates experimental autoimmune encephalomyelitis in MOG-specific TCR transgenic mice
摘要
Abstract
Objective:To induce an experimental autoimmune encephalomyelitis(EAE)model by MOG-specific TCR trans-genic mice(2D2TCR transgenic mice),and to investigate effect of exogenous ERMAP on T cells in spleen of MOG35-55-induced 2D2TCR transgenic mice.Methods:EAE models were established in two groups of 2D2TCR transgenic mice(Control-Ig treatment for control group and ERMAP-Ig fusion protein treatment for experimental group),with 9 mice per group.Severity of spinal cord injury of MOG35-55-induced EAE in mice was assessed based on daily clinical scores(DAI),HE and LFB staining results;autoreactive T cells(CD4+Vα3.2+Vβ11+),T cell proliferation activation indicators CD69(CD4+Vα3.2+Vβ11+CD69+)and Ki67(CD4+Vα3.2+Vβ11+Ki67+),Treg(CD4+Vα3.2+Vβ11+CD25+Foxp3+)and Th17 cells(CD4+Vα3.2+Vβ11+IL-17A+)in spleen were detected by flow cytome-try;IL-17A,IL-6,IFN-γ and TGF-β expressions in spinal cord tissues were detected by qRT-PCR.Results:In MOG35-55-induced 2D2TCR transgenic mouse EAE model,ERMAP-Ig fusion protein treatment group showed milder inflammatory infiltration and demye-lination in spinal cord,decreased proportion of autoreactive T cells,decreased proportion of activated and proliferating T cells,increased proportion of Treg,inhibition of Th17 cell differentiation,less inflammatory cell aggregation and cytokine production,and increased expression of anti-inflammatory factors in spinal cord.Conclusion:ERMAP may be involved in development of EAE in 2D2TCR transgenic mice by inhibiting T cell proliferative activation and promoting Treg cell production.关键词
红细胞膜相关蛋白/自身反应性T细胞/2D2TCR/实验性自身免疫性脑脊髓炎Key words
ERMAP/Autoreactive T cells/2D2TCR/Experimental autoimmune encephalomyelitis分类
基础医学引用本文复制引用
朱婕,宋雯茜,陈柯竹,李远迪,高杰,胡蓉,苏敏..ERMAP改善MOG特异性TCR转基因小鼠实验性自身免疫性脑脊髓炎[J].中国免疫学杂志,2025,41(6):1344-1349,6.基金项目
国家自然科学基金(82171343,82060151) （82171343,82060151）
贵州省高等学校自身免疫病研究重点实验室(黔教技[2023]016号) （黔教技[2023]016号）
贵州省科技厅项目(黔科合基础-ZK[2022]一般344). （黔科合基础-ZK[2022]一般344）
