白细胞介素-9诱导破骨细胞分化在包虫病骨侵蚀中的机制研究OA

Objective To investigate the role of interleukin-9(IL-9)in the invasive growth of bone cystic tissue with cystic echinococcosis and its molecular mechanisms.Methods Paraffin sections of bone cystic tissues from 8 patients with cystic echinococcosis were collected,and paraffin sections of affected knee joint tissues from 8 patients with osteoarthritis were selected as controls.qPCR was used to detect the expression levels of IL-9 mRNA in bone cystic tissues and osteoarthritis bone tissues.RAW264.7 cells were divided into the Undifferentia-tion group(Group 1),the MCSF group(Group 2),the MCSF+RANKL group(Group 3),the MCSF+RANKL+echinococcosis antigen B group(Group 4),and the MCSF+RANKL+IL-9 group(Group 5).qPCR was used to detect the expression levels of TRAP and cathepsin K mRNA in cells of each group.Western blot was used to detect the expression levels of p-p65,p65,nuclear factor of activated T-cells 1(NFATc1),phosphorylated signal transducer and activator of transcription 3(p-STAT3),signal transducer and activator of transcription 3(STAT3),phosphorylated extracellular signal-regulated kinase 1/2(p-ERK1/2)and extracellular signal-regulated kinase 1/2(ERK1/2)in cells of each group.Results The expression level of IL-9 mRNA was higher in bone cystic tissues than that in osteoarthritis bone tissues(P<0.05).Compared with Group 1,the expression levels of TRAP and cathepsin K mRNA in Group 2,Group 3,Group 4 and Group 5 were significantly increased(P<0.05),as well as the levels of p-p65,p-STAT3 and p-ERK1/2,and the expression levels of NFATc1(P<0.05);Compared with Group 3,the above indexes in Group 5 were all increased(P<0.05).Conclusion The expression level of IL-9 is up-regulated in bone cystic tissues,and it enhances the induction of osteoclast differentiation by up-regulating the nuclear factor-κB(NF-κB)/STAT3/ERK1/2 signaling pathway,which may play an important role in bone erosion of bone echinococcosis.