中国农业科技导报2025，Vol.27Issue(6)：136-147,12.DOI:10.13304/j.nykjdb.2024.0157
槐米制备槲皮素及其与粘杆菌素联用对沙门氏菌的作用
Preparation of Quercetin from Sophora japonica and Its Effect Together with Colistin on Salmonella spp
摘要
Abstract
Quercetin is a flavonoid compound with various biological activities,mostly existing in the form of glycosides in plants.Sophora japonica is an ideal raw material for preparing quercetin,with advantages such as wide planting area and low price.This study investigated the synergistic antibacterial effects of quercetin against Salmonella spp.that combined with colistin.The quercetin was prepared from the Sophora japonica buds Aspergillus niger fermentation and isolated from the semi-solid fermentation products using ultrasonic extraction methods,purified and structurally identified using HPLC,ESI-MS and NMR methods.The antibacterial activities of quercetin and colistin,both individually and in combination,against Salmonella were evaluated using dilution and checkerboard methods.The results showed that the highest product of quercetin was 73.7 mg·g-1 after fermentation for 96 h and the purity reached to 98%after purification and identification.After evaluation of antibacterial activity,the minimum inhibitory concentrations(MICs)of colistin against S.typhimurium,S.pullorum 1789 and 520 were 2,8,and 4 μg·mL-1,respectively,while the MICs of quercetin against the mentioned strains were all above 500 μg·mL-1.When quercetin was used together with colistin,the synergistic antibacterial effects were observed against above Salmonella strains,particularly against S.pullorum 1789 and 520,with fractional inhibitory concentration index(FICI)values below 0.5,and the MIC value of colistin were reduced to 1 μg·mL-1.The study demonstrated the synergistic inhibitory effect of quercetin prepared from Sophora japonica through A.niger fermentation combined with colistin against Salmonella,providing a scientific basis for developing new antibacterial combination strategies.关键词
槲皮素/槐米/黑曲霉/粘杆菌素/沙门氏菌/协同抑制Key words
quercetin/Sophora japonica/Aspergillus niger/Salmonella/colistin/synergistic inhibition分类
农业科技引用本文复制引用
熊小艳,田丹丹,弓雨欣,乔宇,徐小轻,何孟欣,石波,彭晴..槐米制备槲皮素及其与粘杆菌素联用对沙门氏菌的作用[J].中国农业科技导报,2025,27(6):136-147,12.基金项目
中国农业科学院科技创新工程项目(CAAS-ASTIP-2023-IFR-09) （CAAS-ASTIP-2023-IFR-09）
国家自然科学基金面上项目(32072773). （32072773）
