中国免疫学杂志2025，Vol.41Issue(6)：1358-1364,7.DOI:10.3969/j.issn.1000-484X.2025.06.017
脱细胞化细胞外基质材料浸提液对RAW264.7巨噬细胞免疫特性的影响
Effect of extracellular matrix extract of decellularized extracellular matrix material on immune characteristics of RAW264.7 macrophages
摘要
Abstract
Objective:To investigate the effects of decellularised extracellular matrix material(dECM)extract on macrophage survival,migration,phagocytosis,pro-inflammatory factor expression and ROS production.Methods:CCK-8 method was used to detect whether the extract of dECM material had cytotoxicity on macrophages;effects of dECM material extracts on macrophage recruit-ment and chemotaxis were examined by Transwell migration assay;effects of dECM material extract on macrophage phagocytosis were detected by pHrodo staining;effects of dECM material extract on expression of pro-inflammatory genes and pro-inflammatory specific functional molecules were detected by RT-qPCR and immunofluorescence staining;detection of the effect of dECM material extracts on macrophage ROS production by the DCFH-DA fluorescent probe approach.Results:Compared with DMEM complete medium,①CCK-8 assay showed that dECM material extract had no toxic effect on macrophages,and could promote the formation of macrophage colonies;②pHrodo staining assay showed that dECM material extract did not affect phagocytosis of macrophages;③Transwell migra-tion assay showed that dECM material extract did not promote macrophage migration;④RT-qPCR results showed that dECM material extracts were able to down-regulate gene expressions of pro-inflammatory cytokines and specific functional molecules;⑤Flow cytome-try results showed that dECM material extract could reduce the production of ROS by macrophages.dECM material extract had excel-lent biocompatibility for macrophages.Conclusion:dECM material extract is non-toxic to macrophages and is able to reduce macro-phage pro-inflammatory gene expression and ROS production.关键词
dECM/脱细胞化细胞外基质/巨噬细胞极化/ROS/炎症Key words
dECM/Decellularized extracellular matrix/Macrophage polarization/ROS/Inflammation分类
基础医学引用本文复制引用
马炬文,张航,王蕴雯,王永为,宋鸿儒,高亚贤..脱细胞化细胞外基质材料浸提液对RAW264.7巨噬细胞免疫特性的影响[J].中国免疫学杂志,2025,41(6):1358-1364,7.基金项目
河北省自然科学基金精准医学联合基金细化项目(H2021406066) （H2021406066）
河北省自然科学基金中医药联合基金培育项目(H2023406057). （H2023406057）
