中国免疫学杂志2025，Vol.41Issue(6)：1371-1375,5.DOI:10.3969/j.issn.1000-484X.2025.06.019
姜黄素调节JAK2/STAT3信号通路对痤疮小鼠Th17/Treg细胞免疫平衡的影响
Effect of curcumin on immune balance of Th17/Treg cells in acne mice by regulating JAK2/STAT3 signaling pathway
摘要
Abstract
Objective:To investigate the effect of curcumin on Th17/Treg cell immune balance in acne mice by regulating the JAK2/STAT3 signaling pathway.Methods:An acne mouse model was established by injecting propionibacterium suspension.The suc-cessfully established mice were randomly separated into the acne group,low(curcumin-L,50 mg/kg curcumin),medium(curcumin-M,100 mg/kg curcumin),high dose(curcumin-H,150 mg/kg curcumin)curcumin groups,and curcumin-H+JAK2/STAT3 pathway activator-olivelin group(150 mg/kg curcumin+1.0 mg/kg colivelin),with healthy mice as the control group,after intervention,the mice were scored for skin lesions;blood was taken from the orbit,ELISA and flow cytometry were applied to detect serum levels of IL-6 and IL-10,and the ratios of Th17 and Treg cells;HE was applied to detect pathological changes in auricle tissue;Western blot was applied to detect the expressions of p-JAK2,p-STAT3,JAK2,STAT3,Treg transcription factor forked transcription factor 3(FOXP3)and Th17 transcription factor retinoic acid related solitary receptor(RORγt)proteins in auricle tissue.Results:Compared with the control group,the pathological damage in the acne group was severe,the skin lesion and pathological scores,IL-6 level,Th17 cells ratio,p-JAK2/JAK2,p-STAT3/STAT3,and RORγt expression were obviously increased,the IL-10 level,Treg cells ra-tio,and FOXP3 expression were obviously reduced(P<0.05);compared with the acne group,the skin lesion and pathological scores,IL-6 level,Th17 cells ratio,p-JAK2/JAK2,p-STAT3/STAT3 and RORγt expression in curcumin-L,curcumin-M,and curcumin-H groups were obviously reduced,the IL-10 level,Treg cells ratio,and FOXP3 expression were obviously increased(P<0.05);com-pared with the curcumin-H group,the skin lesion and pathological scores,IL-6 level,Th17 cells ratio,p-JAK2/JAK2,p-STAT3/STAT3,and RORγt expression in curcumin-H+colivelin group were obviously increased,the IL-10 level,Treg cells ratio,and FOXP3 expression were obviously reduced(P<0.05).Conclusion:Curcumin can improve the immune balance of Th17/Treg cells in acne mice,which may be related to the inhibition of the JAK2/STAT3 signaling pathway.关键词
姜黄素/痤疮/Th17/Treg细胞免疫平衡/JAK2/STAT3信号通路Key words
Curcumin/Acne/Th17/Treg cell immune balance/JAK2/STAT3 signaling pathway分类
医药卫生引用本文复制引用
李知,陈素红,缪飞,许建新..姜黄素调节JAK2/STAT3信号通路对痤疮小鼠Th17/Treg细胞免疫平衡的影响[J].中国免疫学杂志,2025,41(6):1371-1375,5.基金项目
2020年福建省卫生健康科技计划项目(2020GGB050). （2020GGB050）
