中国免疫学杂志2025，Vol.41Issue(6)：1383-1387,5.DOI:10.3969/j.issn.1000-484X.2025.06.021
TLR4/ERK1/2信号通路在孕酮调节蜕膜基质细胞IL-8表达中的作用
Role of TLR4/ERK1/2 signaling pathway in progesterone regulating IL-8 expression in decidual stromal cells
摘要
Abstract
Objective:To explore the mechanism of TLR4/ERK1/2 signaling pathway in progesterone regulating expression of IL-8 in decidual stromal cells(DSCs).Methods:Human DSCs on early pregnancy were isolated and cultured in vitro.The cells were treated with 0.01 μmol/L,0.1 μmol/L and 1 μmol/L progesterone,respectively.Expressions of IL-8,TLR4,ERK1/2 and p-ERK1/2 were detected by Western blot.TLR4 inhibitor TAK-242 and ERK1/2 inhibitor U0126 were further treated,and expression of IL-8 in progesteron+inhibitor group,progesterone group and control group was detected by Western blot.Results:With the increase of pro-gesterone concentration,expression of IL-8 in DSCs decreased gradually.Expressions of DSCs IL-8,TLR4 and p-ERK1/2 in 0.01 μmol/L progesterone group were lower than those in control group(P=0.035 8,P=0.019 4 and P=0.047 1).Expressions of IL-8,TLR4 and p-ERK1/2 in DSCs in 0.1 μmol/L progesterone group were significantly lower than those in control group(P=0.003 5,P=0.004 0 and P=0.009 9).Expressions of IL-8,TLR4 and p-ERK1/2 in DSCs in 1 μmol/L progesterone group were significantly lower than those in control group(P=0.003 3,P=0.001 6 and P=0.005 0).Expression of IL-8 in progesterone+TAK-242 group was signifi-cantly lower than that in progesterone group(P=0.009 1),and expression of IL-8 in progesterone+U0126 group was significantly lower than that in progesterone group(P=0.004).Conclusion:TLR4/ERK1/2 signaling pathway is involved in the physiological process of progesterone regulating expression of IL-8 in DSCs,and progesterone reduces IL-8 expression by inhibiting expression of TLR4 and phosphorylation activation of ERK1/2.关键词
TLR4/ERK1/2信号通路/孕酮/蜕膜基质细胞/IL-8Key words
TLR4/ERK1/2 signaling pathway/Progesterone/Decidual stromal cells/IL-8分类
医药卫生引用本文复制引用
赵紫薇,李娜,杨美霞,宫晓玲,栾兆进..TLR4/ERK1/2信号通路在孕酮调节蜕膜基质细胞IL-8表达中的作用[J].中国免疫学杂志,2025,41(6):1383-1387,5.基金项目
内蒙古自治区自然科学基金项目(2022MS08037,2022QN08016,2021MS08056) （2022MS08037,2022QN08016,2021MS08056）
包头医学院科学研究基金(BYJJ-BSJJ202205). （BYJJ-BSJJ202205）
