中国农业科技导报2025，Vol.27Issue(6)：229-239,11.DOI:10.13304/j.nykjdb.2024.0675
长期施肥对葡萄土壤细菌群落多样性及ARGs分布的影响
Effect of Long-term Fertilization on Diversity of Bacterial Community and Distribution of ARGs in Grape Soil
摘要
Abstract
To examine the influence of long-term fertilization on the diversity of microbial community and the evolution of antibiotic resistance genes(ARGs)in grape soil,the grape soil continuously fertilized for 5 years was as material,with an adjacent plot of uncultivated and unfertilized soil as control(CK).Metagenomic sequencing technology and analysis of the physicochemical properties of the soil were employed to conduct a comprehensive investigation into the impact of long-term fertilization on the diversity of the microbial community and the distribution of ARGs in grape soil.The results revealed that,compared with CK,long-term fertilization resulted in a notable reduction of soil pH and a substantial alteration in soil nutrient composition.The contents of soil organic matter,total nitrogen and total potassium increased by 236.0%,243.3%and 7.4%,respectively.Furthermore,the contents of alkali-soluble nitrogen and ammonium nitrogen increased significantly by 9.61 and 9.28 times,respectively.Additionally,the soil microbial biomass carbon and microbial biomass nitrogen increased by 19.2%and 8.53 times,respectively.Furthermore,compared with CK,long-term fertilization resulted in the addition of 218 new specific drug resistance genes and the reduction of 86 natural drug resistance genes in grape soil.The relative abundances of the genera Nitrospira and Anaeromyxobacter were decreased by 11.4%and 3.1%,respectively,as a consequence of the influence of physical and chemical factors such as soil pH,organic matter,total nitrogen and total potassium.The relative abundances of the genera Pseudogracilibacillus,Staphylococcus,Pseudolabrys and Virgibacillus increased by 10.5%,6.1%,0.6%and 5.4%,respectively.These changes in dominant bacterial groups were closely related to ARGs and played a role in regulating the overall function and metabolism of the grape soil microbiota.In conclusion,long-term fertilization could markedly enhance the physical and chemical properties of grape soil,while also should exert a profound influence on the diversity of soil microbial community and the number of ARGs.关键词
葡萄土壤/长期施肥/土壤微生物群落/抗生素抗性基因(ARGs)/分布规律Key words
grape soil/long-term fertilization/soil microbial community/antibiotic resistance genes(ARGs)/distribution patterns分类
农业科学引用本文复制引用
王会来,李帅,王寅,吴东涛,马嘉伟,叶正钱,池永清,王美..长期施肥对葡萄土壤细菌群落多样性及ARGs分布的影响[J].中国农业科技导报,2025,27(6):229-239,11.基金项目
浙江农林大学人才启动项目(2023LFR014). （2023LFR014）
